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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(3) [July 2012] >
| Title: | Somatic embryogenesis in Jatropha curcus L. using cotyledonary leaves |
| Authors: | Saxena, Sonal
Sharma, Amit
Sardana, Jyoti
Sharma, Madan Mohan
Batra, Amla |
| Keywords: | Cotyledonary shape
Embryo
Embryogenic callus
Globular
Heart shape
Jatropha curcus
Torpedo shape |
| Issue Date: | Jul-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | An efficient
protocol has been developed for high frequency plant regeneration through
somatic embryogenesis in the biodiesel plant, Jatropha curcus. Cotyledonary leaves were used for the development
of the embryogenic callus on MS medium with
0.2 mg/L indole-3-acetic acid (IAA), which developed within
3-4 wk. Later, somatic embryos developed on MS medium with IAA (0.2 mg/L) along
with 6-benzyl aminopurine (BAP; 1.5 mg/L). Fist, globular embryo clumps were
seen within 3-4 wk and then they got transformed into heart, torpedo and
cotyledonary shaped embryos on the same medium after 6 wk. On further
subculture on the same medium, embryos germinated with shoot and root
primordia, which ultimately gave rise to complete plantlets within 4-5 wk. The
pantlets were hardened and transferred to the soil. Around 50% plantlets
survived in the soil. |
| Page(s): | 348-351 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(3) [July 2012]
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