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Title: Somatic embryogenesis in <i style="mso-bidi-font-style:normal">Jatropha curcus </i>L. using cotyledonary leaves
Authors: Saxena, Sonal
Sharma, Amit
Sardana, Jyoti
Sharma, Madan Mohan
Batra, Amla
Keywords: Cotyledonary shape
Embryogenic callus
Heart shape
<i>Jatropha curcus</i>
Torpedo shape
Issue Date: Jul-2012
Publisher: NISCAIR-CSIR, India
Abstract: An efficient protocol has been developed for high frequency plant regeneration through somatic embryogenesis in the biodiesel plant, <i style="mso-bidi-font-style: normal">Jatropha curcus</i>. Cotyledonary leaves were used for the development of the embryogenic callus on MS medium with 0.2 mg/L indole-3-acetic acid (IAA), which developed within 3-4 wk. Later, somatic embryos developed on MS medium with IAA (0.2 mg/L)<sup> </sup>along with 6-benzyl aminopurine (BAP; 1.5 mg/L). Fist, globular embryo clumps were seen within 3-4 wk and then they got transformed into heart, torpedo and cotyledonary shaped embryos on the same medium after 6 wk. On further subculture on the same medium, embryos germinated with shoot and root primordia, which ultimately gave rise to complete plantlets within 4-5 wk. The pantlets were hardened and transferred to the soil. Around 50% plantlets survived in the soil.
Description: 348-351
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.11(3) [July 2012]

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