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Title: Somatic embryogenesis in Jatropha curcus L. using cotyledonary leaves
Authors: Saxena, Sonal
Sharma, Amit
Sardana, Jyoti
Sharma, Madan Mohan
Batra, Amla
Keywords: Cotyledonary shape;Embryo;Embryogenic callus;Globular;Heart shape;Jatropha curcus;Torpedo shape
Issue Date: Jul-2012
Publisher: NISCAIR-CSIR, India
Abstract: An efficient protocol has been developed for high frequency plant regeneration through somatic embryogenesis in the biodiesel plant, Jatropha curcus. Cotyledonary leaves were used for the development of the embryogenic callus on MS medium with 0.2 mg/L indole-3-acetic acid (IAA), which developed within 3-4 wk. Later, somatic embryos developed on MS medium with IAA (0.2 mg/L) along with 6-benzyl aminopurine (BAP; 1.5 mg/L). Fist, globular embryo clumps were seen within 3-4 wk and then they got transformed into heart, torpedo and cotyledonary shaped embryos on the same medium after 6 wk. On further subculture on the same medium, embryos germinated with shoot and root primordia, which ultimately gave rise to complete plantlets within 4-5 wk. The pantlets were hardened and transferred to the soil. Around 50% plantlets survived in the soil.
Page(s): 348-351
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.11(3) [July 2012]

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