NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(3) [July 2012] >


Title: In vitro shoot multiplication from nodal explants of Boucerosia diffusa Wight—An endemic medicinal plant
Authors: Ramadevi, T
Ugraiah, A
Pullaiah, T
Keywords: Apocynaceae
Boucerasia diffusa
In vitro propagation
Plant growth regulators
Issue Date: Jul-2012
Publisher: NISCAIR-CSIR, India
Abstract: In vitro propagation of Boucerosia diffusa Wight (Apocynaceae), an endemic medicinal plant of Western Ghats, (Tamil Nadu, India) was carried out. Nodal explants were cultured on MS medium supplemented with cytokinins [benzyl aminopurine (BAP), kinetin (Kn), thidiazuron (TDZ) & zeatin] either individually or in combinations. Shoot buds proliferated on MS medium+BAP (8.87 µM) and repeated subculture on MS+BAP (8.87 µM)+TDZ (2.27 µM) promoted the shoot multiplication. The regenerated shoots were then rooted on MS+NAA (2.69 µM). The micropropagated planlets were hardened with the survival success rate of 56%. The developed micropropagation protocol would help ex situ conservation of this endemic species.
Page(s): 344-347
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.11(3) [July 2012]

Files in This Item:

File Description SizeFormat
IJBT 11(3) 344-347.pdf119.66 kBAdobe PDFView/Open
 Current Page Visits: 857 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 620660 since 06-Feb-2009  Last updated on 13-Nov-2014Webmaster: nopr@niscair.res.in