Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/14580
Title: <i style="mso-bidi-font-style:normal"><span style="font-size:13.0pt;mso-bidi-font-size:10.0pt" lang="EN-GB">In vitro</span></i><span style="font-size:13.0pt;mso-bidi-font-size:10.0pt" lang="EN-GB"> shoot multiplication from nodal explants of <i style="mso-bidi-font-style:normal">Boucerosia diffusa</i> Wight—An endemic medicinal plant </span>
Authors: Ramadevi, T
Ugraiah, A
Pullaiah, T
Keywords: Apocynaceae
<i style="mso-bidi-font-style:normal"><span style="font-size:11.0pt;mso-bidi-font-size:9.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";letter-spacing:-.2pt;mso-ansi-language: EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">Boucerasia diffusa</span></i>
<i style="mso-bidi-font-style:normal"><span style="font-size:11.0pt;mso-bidi-font-size:9.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";letter-spacing:-.2pt;mso-ansi-language: EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">In vitro</span></i><span style="font-size:11.0pt;mso-bidi-font-size:9.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";letter-spacing:-.2pt;mso-ansi-language: EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB"> propagation</span>
Plant growth regulators
Issue Date: Jul-2012
Publisher: NISCAIR-CSIR, India
Abstract: <i style="mso-bidi-font-style:normal"><span style="mso-bidi-font-size:9.0pt;letter-spacing:-.1pt" lang="EN-GB">In vitro</span></i><span style="mso-bidi-font-size:9.0pt;letter-spacing:-.1pt" lang="EN-GB"> propagation of <i style="mso-bidi-font-style:normal">Boucerosia diffusa</i> Wight (Apocynaceae), an endemic medicinal plant of Western Ghats, (Tamil Nadu, India) was carried out. Nodal explants were cultured on MS medium supplemented with cytokinins [benzyl aminopurine (BAP), kinetin (Kn), thidiazuron (TDZ) & zeatin] either individually or in combinations. Shoot buds proliferated on MS medium+BAP (8.87 µ<i style="mso-bidi-font-style:normal">M</i>) and repeated subculture on MS+BAP (8.87 µ<i style="mso-bidi-font-style:normal">M</i>)+TDZ (2.27 µ<i style="mso-bidi-font-style:normal">M</i>) promoted the shoot multiplication. The regenerated shoots were then rooted on MS+NAA (2.69 µ<i style="mso-bidi-font-style:normal">M</i>). The micropropagated planlets were hardened with the survival success rate of 56%. The developed micropropagation protocol would help <i style="mso-bidi-font-style:normal">ex situ</i> conservation of this endemic species. </span>
Description: 344-347
URI: http://hdl.handle.net/123456789/14580
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.11(3) [July 2012]

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