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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(3) [July 2012] >
| Title: | In vitro shoot multiplication from nodal explants of Boucerosia diffusa Wight—An endemic medicinal plant |
| Authors: | Ramadevi, T
Ugraiah, A
Pullaiah, T |
| Keywords: | Apocynaceae
Boucerasia diffusa
In vitro propagation
Plant growth regulators |
| Issue Date: | Jul-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In vitro propagation
of Boucerosia diffusa Wight
(Apocynaceae), an endemic medicinal plant of Western Ghats, (Tamil Nadu, India) was
carried out. Nodal explants were cultured on MS medium supplemented with
cytokinins [benzyl aminopurine (BAP), kinetin (Kn), thidiazuron (TDZ) &
zeatin] either individually or in combinations. Shoot buds proliferated on MS
medium+BAP (8.87 µM) and repeated
subculture on MS+BAP (8.87 µM)+TDZ
(2.27 µM) promoted the shoot
multiplication. The regenerated shoots were then rooted on MS+NAA (2.69 µM). The micropropagated planlets were
hardened with the survival success rate of 56%. The developed micropropagation
protocol would help ex situ
conservation of this endemic species.
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| Page(s): | 344-347 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(3) [July 2012]
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