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Title: In vitro shoot multiplication from nodal explants of Boucerosia diffusa Wight—An endemic medicinal plant
Authors: Ramadevi, T
Ugraiah, A
Pullaiah, T
Keywords: Apocynaceae;Boucerasia diffusa;In vitro propagation;Plant growth regulators
Issue Date: Jul-2012
Publisher: NISCAIR-CSIR, India
Abstract: In vitro propagation of Boucerosia diffusa Wight (Apocynaceae), an endemic medicinal plant of Western Ghats, (Tamil Nadu, India) was carried out. Nodal explants were cultured on MS medium supplemented with cytokinins [benzyl aminopurine (BAP), kinetin (Kn), thidiazuron (TDZ) & zeatin] either individually or in combinations. Shoot buds proliferated on MS medium+BAP (8.87 µM) and repeated subculture on MS+BAP (8.87 µM)+TDZ (2.27 µM) promoted the shoot multiplication. The regenerated shoots were then rooted on MS+NAA (2.69 µM). The micropropagated planlets were hardened with the survival success rate of 56%. The developed micropropagation protocol would help ex situ conservation of this endemic species.
Page(s): 344-347
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.11(3) [July 2012]

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