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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(3) [July 2012] >
| Title: | Evaluation of genetic stability of micropropagated plants of three species of Garcinia using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers |
| Authors: | Mohan, Simi
Parthasarathy, Utpala
Asish, G R
Babu, K Nirmal |
| Keywords: | Garcinia
Genetic stability
ISSR
Micropropagation
RAPD |
| Issue Date: | Jul-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Random amplified polymorphic DNA (RAPD) and inter
simple sequence repeat (ISSR) markers
were used to analyze the genetic stability of micropropagated as well as mother
plants of three species of Garcinia, viz., G. gummigutta, G. indica, and
G. tinctoria. Twelve RAPD primers and four ISSR primers generated clear,
distinct and reproducible bands. Total genomic DNA extracted from fresh leaves
of in vitro regenerated as well as mother plants was used for developing
RAPD and ISSR profiles. All RAPD and ISSR profiles from micropropagated plants
were monomorphic and similar to those of the field grown mother plants. No genetic variation
was detected within the micropropagated plants in comparison to their mother
plants. The results indicate that the Garcinia
plants regenerated using micropropagation system standardized at our laboratory
were genetically stable. |
| Page(s): | 341-343 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(3) [July 2012]
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