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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(3) [July 2012] >
| Title: | Isozyme diversity in selected leaf mutants of ‘tossa’ jute (Corchorus olitorius L.) |
| Authors: | Ali, Md Nasim
Ghosh, A
Sasmal, B G
Sarkar, H K
Das, P K |
| Keywords: | Clustering
Esterase
Isozyme diversity
Peroxidase
Tossa jute |
| Issue Date: | Jul-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Electrophoregrams of peroxidase and esterase
were obtained in polyacrylamide gel for 6 leaf mutants (3 natural, 2 X-ray and
1  -ray induced mutants) of ‘tossa’ jute JRO-632 and, on their basis, dendrograms
were generated to ascertain relationship between/among mutants. Two unique slow
moving peroxidase bands (presumably of high mol wt), numbered as band 1 and 2, were
helpful to identify two X-ray mutants from the -ray derived mutant and one
specific natural mutant from other two natural mutants. The presence of
esterase band 1 in
only -ray induced mutant and not in X-ray induced mutants suggested the
uniqueness of this slow moving high mol wt band that differentiates these two
groups of mutants of different origin. Thus, these bands of high mol wt in
peroxidase and esterase could serve as distinguishing reference band. Moreover,
band of esterase numbered as 7 was expressed only in one of the X-ray mutant
and, thus, differentiates between the both mutants. The cluster analysis from
constructed dendrograms on the basis of 60% similarity revealed only two groups
for peroxidase and five clusters for esterase. |
| Page(s): | 333-336 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(3) [July 2012]
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