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Title: Polymorphism in TLR4 gene and its association with mastitis in Murrah buffaloes
Authors: Gulhane, A B
Sangwan, M L
Keywords: Mastitis;Murrah buffalo;PCR-RFLP;TLR4
Issue Date: Jul-2012
Publisher: NISCAIR-CSIR, India
Abstract: Genomic DNA was isolated from blood samples, collected from 60 unrelated Murrah buffaloes (Bubalus bubalis) including 30 animals suffering/suffered from mastitis. Exon 3 of TLR4 gene was amplified from genomic DNA, which gave a PCR product of 386 bp. The digestion of 386 bp PCR product of TLR4 gene with StyI restriction enzyme detected two genotypes, viz., aa and ab with frequencies 0.57 and 0.43, respectively and alleles a and b were observed with frequencies 0.78 and 0.22, respectively. There was significant (P≤0.05) difference in the genotypic frequencies of these two genotypes in healthy and mastitis animals. The frequency of aa genotype was significantly higher (P≤0.05) in healthy animals, which indicates the association of aa genotype with resistance to mastitis. In phylogenetic analysis, TLR gene of B. bubalis was found more closely related to goat and sheep than taurine and zebu cattle.
Page(s): 330-332
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.11(3) [July 2012]

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