NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(3) [July 2012] >


Title: Polymorphism in TLR4 gene and its association with mastitis in Murrah buffaloes
Authors: Gulhane, A B
Sangwan, M L
Keywords: Mastitis
Murrah buffalo
PCR-RFLP
TLR4
Issue Date: Jul-2012
Publisher: NISCAIR-CSIR, India
Abstract: Genomic DNA was isolated from blood samples, collected from 60 unrelated Murrah buffaloes (Bubalus bubalis) including 30 animals suffering/suffered from mastitis. Exon 3 of TLR4 gene was amplified from genomic DNA, which gave a PCR product of 386 bp. The digestion of 386 bp PCR product of TLR4 gene with StyI restriction enzyme detected two genotypes, viz., aa and ab with frequencies 0.57 and 0.43, respectively and alleles a and b were observed with frequencies 0.78 and 0.22, respectively. There was significant (P≤0.05) difference in the genotypic frequencies of these two genotypes in healthy and mastitis animals. The frequency of aa genotype was significantly higher (P≤0.05) in healthy animals, which indicates the association of aa genotype with resistance to mastitis. In phylogenetic analysis, TLR gene of B. bubalis was found more closely related to goat and sheep than taurine and zebu cattle.
Page(s): 330-332
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.11(3) [July 2012]

Files in This Item:

File Description SizeFormat
IJBT 11(3) 330-332.pdf205.03 kBAdobe PDFView/Open
 Current Page Visits: 371 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 558493 since 06-Feb-2009  Last updated on 30-Jul-2014Webmaster: nopr@niscair.res.in