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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(3) [July 2012] >
| Title: | Polymorphism in TLR4 gene and its association with mastitis in Murrah buffaloes |
| Authors: | Gulhane, A B
Sangwan, M L |
| Keywords: | Mastitis
Murrah buffalo
PCR-RFLP
TLR4 |
| Issue Date: | Jul-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Genomic DNA was isolated from blood samples, collected from 60 unrelated
Murrah buffaloes (Bubalus bubalis)
including 30 animals suffering/suffered from mastitis. Exon 3 of TLR4 gene was amplified
from genomic DNA, which gave a PCR product of 386 bp. The
digestion of 386 bp PCR product of TLR4 gene with StyI restriction
enzyme detected two genotypes, viz., aa and ab with frequencies 0.57 and 0.43, respectively and
alleles a and b were
observed with frequencies 0.78 and 0.22, respectively. There was significant (P≤0.05)
difference in the genotypic frequencies of these two genotypes in healthy and
mastitis animals. The frequency of aa genotype was
significantly higher (P≤0.05) in healthy animals,
which indicates the association of aa genotype with resistance to mastitis.
In phylogenetic analysis, TLR gene of
B. bubalis was found more closely
related to goat and sheep than taurine and zebu cattle. |
| Page(s): | 330-332 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(3) [July 2012]
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