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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(3) [July 2012] >
| Title: | Inducive effect of L-methionine in transformation of L-tyrosine to L-Dopa and tyrosinase production by Streptomyces sp. VRS9 |
| Authors: | Patil, Santosh
Rathod, Vandana
Ranganath, E |
| Keywords: | Inducers
L-Dopa
Parkinson`s disease
Streptomyces sp.
Tyrosinase |
| Issue Date: | Jul-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A total of 52 isolates of actinomycetes were isolated
from dry land soils of Gulbarga region, Karnataka, India.
The actinomycete designated as isolate VRS9 was selected for its high tyrosinase
(monophenolase) activity (67 U/mL) in standard medium. The taxonomical
properties of the isolate were examined according to the International
Streptomyces Project (ISP) scheme and the isolate VRS9 was assigned to the
Genus Streptomyces. The inducive effect
of L-methionine on transformation of L-tyrosine to L-Dopa
(3,4-dihydroxyphenyl-L-alanine) under submerged conditions using Streptomyces sp. VRS9 was investigated. The fermentation was carried at 35°C and pH 8.0, which were optimum physical
conditions applied to test the effect of methionine on tyrosinase and L-Dopa
production. Studies showed 1.15 and 0.52 folds higher production of tyrosinase
and L-Dopa, respectively as compared to bioreaction conducted without addition
of methionine. VRS9 gave the maximum L-Dopa production (0.90 mg/mL) and
tyrosinase activity (289 U/mL) at 10 mM
concentration of methionine added during the beginning of log phase. |
| Page(s): | 320-325 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(3) [July 2012]
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