Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/14574
Title: <span style="font-size:15.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Inducive effect of L-methionine in transformation of L-tyrosine to L-Dopa and tyrosinase production by <i style="mso-bidi-font-style:normal">Streptomyces </i>sp. VRS9</span>
Authors: Patil, Santosh
Rathod, Vandana
Ranganath, E
Keywords: Inducers
L-Dopa
Parkinson`s disease
<i style="mso-bidi-font-style:normal"><span style="font-size:11.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB">Streptomyces </span></i><span style="font-size:11.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB">sp<i style="mso-bidi-font-style: normal">.</i></span>
Tyrosinase
Issue Date: Jul-2012
Publisher: NISCAIR-CSIR, India
Abstract: A total of 52 isolates of actinomycetes were isolated from dry land soils of Gulbarga region, Karnataka, India. The actinomycete designated as isolate VRS9 was selected for its high tyrosinase (monophenolase) activity (67 U/mL) in standard medium. The taxonomical properties of the isolate were examined according to the International Streptomyces Project (ISP) scheme and the isolate VRS9 was assigned to the Genus <i style="mso-bidi-font-style:normal">Streptomyces. </i>The inducive effect of L-methionine on transformation of L-tyrosine to L-Dopa (3,4-dihydroxyphenyl-L-alanine) under submerged conditions using <i style="mso-bidi-font-style:normal">Streptomyces </i>sp<i style="mso-bidi-font-style: normal">.</i> VRS9 was investigated. The fermentation was carried at 35°C and <i style="mso-bidi-font-style:normal">p</i>H 8.0, which were optimum physical conditions applied to test the effect of methionine on tyrosinase and L-Dopa production. Studies showed 1.15 and 0.52 folds higher production of tyrosinase and L-Dopa, respectively as compared to bioreaction conducted without addition of methionine. VRS9 gave the maximum L-Dopa production (0.90 mg/mL) and tyrosinase activity (289 U/mL) at 10 m<i style="mso-bidi-font-style:normal">M</i> concentration of methionine added during the beginning of log phase.
Description: 320-325
URI: http://hdl.handle.net/123456789/14574
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.11(3) [July 2012]

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