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|Title:||In vitro regeneration of Begonia rubrovenia var. meisneri C.B. Clarke— A rare and endemic ornamental plant of Meghalaya, India|
Bhowmik, Sudipta Shekhar Das
|Keywords:||In vitro techniques;Leaf and petiole sections;Shoot multiplication;Threatened plant|
|Abstract:||In vitro plant regeneration from leaf and petiole explants of Begonia rubrovenia meisneri C.B. Clarke, an endemic and rare plant of Meghalaya, has been established. Multiple shoots were induced on Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzyl aminopurine (BAP) and thidiazuron (TDZ). In vitro produced leaf and petiole sections (secondary explants) exhibited enhanced shoot proliferation in MS medium supplemented with either BAP or TDZ in different concentrations (0.05-5.0 mg/L). A maximum, 65 shoots/petiole were formed on MS+0.1 mg/L TDZ. The in vitro-raised shoots formed roots in MS medium containing 0.1 mg/L indole 3-acetic acid (IAA), with a maximum of 13.8 roots/shoot. About 73.33% of the 500 plantlets transferred acclimatized successfully within 4 wk in a glasshouse. On being transferred to the field, all the acclimatized plantlets survived after 8 wk.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.11(3) [July 2012]|
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