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Title: <i style="mso-bidi-font-style:normal">In vitro</i> propagation and conservation of<i style="mso-bidi-font-style:normal"> Swertia bimaculata</i> Hook.f. & Thoms.
Authors: Dafadar, Arpita
Jha, Timir Baran
Keywords: Chromosomal database
<i style="mso-bidi-font-style:normal"><span style="font-size:9.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB">Swertia bimaculata</span></i>
Shoot multiplication
Issue Date: Jul-2012
Publisher: NISCAIR-CSIR, India
Abstract: <i style="mso-bidi-font-style:normal">Swertia bimaculata</i> Hook.f. & Thoms., a less bitter species of the genus <i style="mso-bidi-font-style:normal">Swertia</i>, is phytochemically important for its bioactive compounds (xanthones). Flower buds and immature capsules were collected from <i style="mso-bidi-font-style:normal">in vivo</i> donor plants from South Sikkim during the month of September 2009. Three to five per cent (3-5%) seeds within the intact or split capsule germinated aseptically in full strength Murashige and Skoog’s (MS) medium with low concentration of either benzylaminopurine (BA; 2.22 µ<i style="mso-bidi-font-style:normal">M</i>) or kinetin (Kn; 2.32 <span style="font-family:Symbol;mso-ascii-font-family:" times="" new="" roman";="" mso-hansi-font-family:"times="" roman";mso-char-type:symbol;mso-symbol-font-family:="" symbol"="" lang="EN-GB">m<i>M</i>). <i style="mso-bidi-font-style:normal">In vitro</i> grown shoot tips were used as primary explants. Shoot multiplication was obtained on half-strength MS medium (BM) with 3% (w/v) sucrose and with different combinations and concentration of BA (2.22-4.44 µ<i style="mso-bidi-font-style: normal">M</i>), Kn (2.32-4.65 µ<i style="mso-bidi-font-style:normal">M</i>) and 1-napthalene acetic acid (NAA; 0.54 µ<i style="mso-bidi-font-style:normal">M</i>). However, the best response with 15.6 shoots per explants was obtained on BM with 2.22 µ<i style="mso-bidi-font-style:normal">M</i> BA, 2.32 µ<i style="mso-bidi-font-style:normal">M</i> Kn and 0.54 µ<i style="mso-bidi-font-style: normal">M</i> NAA. The number of shoots was further increased to 20.6 on addition of 10 m<i style="mso-bidi-font-style:normal">M</i> KNO<sub>3 </sub>in the medium. About 100% healthy root induction on isolated shoots was achieved on BM devoid of any plant growth regulator (PGR) within 5 wk. Complete rooted plantlets were successfully hardened and transplanted in the soil with 80-90% survival rate. Meiotic study from flower buds of donor plants and mitotic chromosome study from regenerated plants revealed n=13 bivalents and 2n=26 chromosomes respectively. The protocol of <i style="mso-bidi-font-style:normal">in vitro</i> propagation and chromosomal database developed for the first time in an unexplored <i style="mso-bidi-font-style:normal">S. bimaculata</i> is highly efficient, repeatable and can be used for conservation of germplasm. </span>
Description: 295-299
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.11(3) [July 2012]

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