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Title: Rapid in vitro mass multiplication of orchids Aerides maculosa Lindl. and Rhynchostylis retusa (L.) Bl. from immature seeds
Authors: Parab, G V
Krishnan, S
Keywords: Callus induction;Orchid;Plant regeneration;Protocorm-like bodies (PLBs)
Issue Date: Jul-2012
Publisher: NISCAIR-CSIR, India
Abstract: The callus embryoid plant regeneration was developed using immature seeds of Aerides maculosa Lindl. and Rhynchostylis retusa (L.) Bl. Immature capsules of 5-10 wk after pollination (A. maculosa) and 10-20 wk after pollination (R. retusa) were used for callus induction. In both the species, best response of callus induction was observed on Vacin and Went (VW) medium supplemented with 15% coconut water (CW, v/v). In A. maculosa, maximum number of protocorm-like bodies (PLBs; 13.93 per callus) was noted on VW medium supplemented with BA (1 mg L-1)+NAA (1 mg L-1) + 15% CW. While in R. retusa, maximum number of PLBs (15.83 per callus) was noted in Murashige and skoog (MS) medium supplemented with BA (1 mg L-1)+NAA (1 mg L-1)+15% CW. In vitro regenerated plantlets of both the species were acclimatized to green house conditions. The highest survival rate of plantlets (60%) was noted in the combination of charcoal pieces, brick pieces (1:1) and coconut husk.
Page(s): 288-294
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.11(3) [July 2012]

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