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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(3) [July 2012] >
| Title: | Rapid in vitro mass multiplication of orchids Aerides maculosa Lindl. and Rhynchostylis retusa (L.) Bl. from immature seeds |
| Authors: | Parab, G V
Krishnan, S |
| Keywords: | Callus induction
Orchid
Plant regeneration
Protocorm-like bodies (PLBs) |
| Issue Date: | Jul-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The callus embryoid plant regeneration was
developed using immature seeds of Aerides maculosa Lindl. and Rhynchostylis
retusa (L.) Bl. Immature capsules of 5-10 wk after pollination (A. maculosa) and 10-20 wk after pollination (R.
retusa) were used for callus
induction. In both the species, best response of callus induction was observed
on Vacin and Went (VW) medium supplemented with 15% coconut water (CW, v/v). In
A. maculosa, maximum number of protocorm-like bodies (PLBs; 13.93 per callus) was noted on VW medium supplemented with BA (1 mg
L-1)+NAA (1 mg L-1) + 15% CW. While in R. retusa, maximum number of PLBs (15.83
per callus) was noted in Murashige and skoog (MS) medium supplemented with BA
(1 mg L-1)+NAA (1 mg L-1)+15% CW. In vitro regenerated plantlets of both
the species were acclimatized to green house conditions. The highest survival
rate of plantlets (60%) was noted in the combination of charcoal pieces, brick
pieces (1:1) and coconut husk. |
| Page(s): | 288-294 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(3) [July 2012]
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