NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(3) [July 2012] >

Title: ARMS-PCR as an alternative, cost effective method for detection of FecB genotype in sheep
Authors: Saste, S R
Ghalsasi, P M
Kataria, R S
Joshi, B K
Mishra, B P
Nimbkar, C
Keywords: ARMS-PCR
Issue Date: Jul-2012
Publisher: NISCAIR-CSIR, India
Abstract: Authors have standardized and report here a simplified amplification refractory mutation system (ARMS)-PCR test as an alternative technique to PCR-RFLP for detection of FecB genotype in sheep. An ARMS-PCR test to detect the FecB mutation in Black Bengal goats has recently been described in the literature. A number of modifications to this technique has been made to simplify and standardize the protocol to use it for sheep DNA, isolated from blood samples on FTA paper. The modifications included reducing the PCR reaction volume to half of original protocol, using a single touchdown annealing temperature and combining two PCR amplicons amplified with different primer pairs (one for each allele of FecB) for each sample prior to loading on the gel. These modifications can make the technique cheaper and more suitable for large scale, rapid FecB genotyping in sheep for use in FecB introgression breeding programmes.
Page(s): 274-279
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.11(3) [July 2012]

Files in This Item:

File Description SizeFormat
IJBT 11(3) 274-279.pdf327.76 kBAdobe PDFView/Open
 Current Page Visits: 110 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 172133 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: