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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(3) [July 2012] >
| Title: | ARMS-PCR as an alternative, cost effective method for detection of FecB genotype in sheep |
| Authors: | Saste, S R
Ghalsasi, P M
Kataria, R S
Joshi, B K
Mishra, B P
Nimbkar, C |
| Keywords: | ARMS-PCR
FecB
Genotyping
PCR-RFLP
Sheep |
| Issue Date: | Jul-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Authors have standardized and report here a simplified amplification refractory mutation system (ARMS)-PCR test as an alternative technique to PCR-RFLP for detection of FecB genotype in sheep. An ARMS-PCR test to detect the FecB
mutation in Black Bengal goats has recently been described in the literature. A
number of modifications to this technique has been made to simplify and
standardize the protocol to use it for sheep DNA, isolated from blood samples
on FTA paper. The modifications included reducing the PCR reaction volume to half of original
protocol, using a single touchdown annealing
temperature and combining two PCR amplicons amplified with different primer
pairs (one for each allele of FecB)
for each sample prior to loading on the gel. These modifications can make the
technique cheaper and more suitable for large scale, rapid FecB genotyping in sheep for use in FecB introgression breeding programmes.
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| Page(s): | 274-279 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(3) [July 2012]
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