Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/14565
Title: Molecular analysis of TLR4 gene and its association with intra-mammary infections in Sahiwal cattle and Murrah buffaloes
Authors: Sentitula
Kumar, Ravinder
Yadav, B R
Keywords: Buffalo
Cattle
Mastitis
PCR-RFLP
TLR4
Issue Date: Jul-2012
Publisher: NISCAIR-CSIR, India
Abstract: Toll-like receptors (TLRs) are a multigene family of pathogen recognition and play a central role in the initiation of<sup> </sup>inflammation response and subsequent adaptive immune system. In the present study, genotypes of TLR4 variants were analysed in mastitic cattle and buffaloes. Genome of selected animals (<i style="mso-bidi-font-style:normal">n</i>=218) comprising of Sahiwal (<i style="mso-bidi-font-style:normal">n</i>=110) cattle and Murrah (<i style="mso-bidi-font-style:normal">n</i>=108) buffaloes on the basis of their health history as well as on the causative agents screening, were analysed for polymorphisms in TLR4 using PCR-RFLP. Molecular analysis of TLR4 gene revealed two alleles (<i>A </i>and <i>B</i>) and three genotypes (<i style="mso-bidi-font-style: normal">AA</i>, <i style="mso-bidi-font-style:normal">BB</i> & <i style="mso-bidi-font-style:normal">AB</i>) in both the breeds. Incidence of mastitis showed significant association of TLR4 variants with non-staphylococci microbes. Genotype <i style="mso-bidi-font-style:normal">BB </i>(Sahiwal, 0.613; Murrah, 0.703)<i style="mso-bidi-font-style:normal"> </i>was found significantly associated with the mastitis irrespective of microbial or other factors, whereas <i style="mso-bidi-font-style:normal">AB</i> predominated (Sahiwal, 0.714; Murrah, 0.772) in unaffected cases. Statistically, association between polymorphism of TLR4 variant and mastitis in both the populations emphasized that genotype<i style="mso-bidi-font-style: normal"> AA</i> were significantly lower than <i style="mso-bidi-font-style: normal">BB</i> (P <0.05). Additionally, <i style="mso-bidi-font-style:normal">AA</i> genotype remained prevalent only in case of mixed microbial infection in Murrah, and streptococcal and mixed microbial infection in case of Sahiwal. Allele <i style="mso-bidi-font-style:normal">A</i> might be favourable for mastitis resistance. Chi square test revealed that the two loci of both the populations were in Hardy-Weinberg equilibrium. The observations of present may be helpful in identifying markers of natural resistance against mastitis that could contribute in its containment programme in cattle and buffaloes.
Description: 267-273
URI: http://hdl.handle.net/123456789/14565
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.11(3) [July 2012]

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