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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(3) [July 2012] >
| Title: | Molecular analysis of TLR4 gene and its association with intra-mammary infections in Sahiwal cattle and Murrah buffaloes |
| Authors: | Sentitula
Kumar, Ravinder
Yadav, B R |
| Keywords: | Buffalo
Cattle
Mastitis
PCR-RFLP
TLR4 |
| Issue Date: | Jul-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Toll-like receptors (TLRs) are a multigene
family of pathogen recognition and play a central role in the initiation of
inflammation response and subsequent adaptive immune system. In the
present study, genotypes of TLR4 variants were analysed in mastitic cattle and
buffaloes. Genome of selected animals (n=218)
comprising of Sahiwal (n=110) cattle
and Murrah (n=108) buffaloes on the
basis of their health history as well as on the causative agents screening,
were analysed for
polymorphisms in TLR4 using PCR-RFLP. Molecular analysis of TLR4 gene revealed
two alleles (A and B) and three genotypes (AA, BB & AB) in both the breeds. Incidence of mastitis showed significant association of TLR4 variants
with non-staphylococci microbes. Genotype BB
(Sahiwal, 0.613; Murrah, 0.703) was
found significantly associated with the mastitis irrespective of microbial or
other factors, whereas AB
predominated (Sahiwal, 0.714; Murrah, 0.772) in unaffected cases.
Statistically, association between polymorphism of TLR4 variant and mastitis in
both the populations emphasized that genotype AA were significantly lower than BB (P <0.05). Additionally, AA
genotype remained prevalent only in case of mixed microbial infection in
Murrah, and streptococcal and mixed microbial infection in case of Sahiwal.
Allele A might be favourable for
mastitis resistance. Chi square test revealed that the two loci of both the
populations were in Hardy-Weinberg equilibrium. The observations of present may be helpful
in identifying markers of natural resistance against mastitis that could
contribute in its containment programme in cattle and buffaloes. |
| Page(s): | 267-273 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(3) [July 2012]
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