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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(3) [July 2012] >
| Title: | RAPD markers for identification of sex in pointed gourd (Trichosanthes dioica Roxb.) |
| Authors: | Kumar, Sanjeev
Singh, B D
Sinha, D P |
| Keywords: | Pointed gourd
RAPD
Sex identification
Trichosanthes |
| Issue Date: | Jul-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Fifty two sexual progeny of pointed gourd (Trichosanthes
dioica Roxb.), raised through embryo culture, were used for identification
of RAPD markers associated with sex expression traits. Genomic DNA from male
and female progeny plants and from a parthenocarpic clone (IIVRPG-105) were
extracted individually and bulked by sex type. Forty-one random decamer primers
were screened with the three bulks and a total of 509 amplification products
were obtained, of which five were found to be associated with sex expression.
The five markers were then tested with individual plant DNA samples, and two
sex-associated RAPD markers were identified. A 1000 bp amplification product
from the primer OPC05 was found to be present only in males and absent in both
the female and parthenocarpic plants. Similarly, a 400 bp amplification product
from the primer OPC14 was found to be present only in female individuals.
Another marker, OPN011030 was also identified, which was
present in male and female plant but was absent in parthenocarpic line. The two
RAPD markers, the male-specific OPC051000 and the
female-specific marker OPC14400 together can reliably differentiate
male and female plants of
T. dioica long before the plants attain maturity and flowering. |
| Page(s): | 251-256 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(3) [July 2012]
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