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Title: <span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Carbon Nanoparticle Toxicity to marine algae <i style="mso-bidi-font-style: normal">Navicula longa</i> and <i style="mso-bidi-font-style:normal">Isochrysis galbana.</i></span>
Authors: Miranda, Ashley A.
Shaikh, Shamshad M.
Sarode, P.R.
Desai, P. V.
Keywords: Carbon
Cell necrosis
Tube like structures
Issue Date: Aug-2012
Publisher: NISCAIR-CSIR, India
Abstract: Present study reports cytotoxicity of Carbon Nanoparticles to <i style="mso-bidi-font-style:normal">Navicula</i> and <i style="mso-bidi-font-style:normal">Isochrysis</i>. Cytotoxicity was determined by morphological changes, cell density count, Acid Phosphatase and Lactate dehydrogenase assays. Carbon nanoparticles (CNPs) promote concentration as well as exposure dependent toxicity to Navicula & Isochrysis, which exhibited cell death, necrotic changes such as cell shrinkage and pycnosis of nucleus. Lactate Dehydrogenase (LDH) & Acid Phosphatase (ACP) activities elevated significantly, in both algae, in a dose and exposure dependant manner. In Navicula and Isochrysis, LDH activity was elevated by 42.3% and 60.7%, and 44.5% and 90.3%, on exposure to 0.05 mg/mL and 1 mg/mL CNPs for 10 days respectively. Similarly, ACP activity in both Navicula and Isochrysis was elevated by 34.6% and 55.4%, and 11.7% and 18.5%, on exposure to 0.05 mg/mL and 1 mg/mL of CNPs for 10 days respectively, as compared to the controls. The occurrence of tube like structures under the influence of CNPs in algae was also observed.
Description: 331-337
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.41(4) [August 2012]

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