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Title: Optimization of media composition for D-amino acid oxidase production by <i>Trigonopsis variabilis </i><span style="mso-bidi-font-style:italic">using biostatistical analysis </span>
Authors: Gupta, Neeraj
Gundampati, Ravi Kumar
Debnath, M
Keywords: D-amino acid oxidase
<i><span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman+FPEF";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Trigonopsis variabilis</span></i>
Response surface methodology
Placket-Burman design
Central composite design
Issue Date: Aug-2012
Publisher: NISCAIR-CSIR, India
Abstract: D-amino acid oxidase (DAAO) is biotechnologically relevant enzyme that is used in various food and pharmaceutical industries. <span style="letter-spacing: -.1pt;mso-ansi-language:EN" lang="EN">DAAO from the yeast <span style="color:black;letter-spacing:-.1pt;mso-ansi-language:EN" lang="EN">Trigonopsis variabilis<span style="letter-spacing:-.1pt;mso-ansi-language: EN" lang="EN"> is an important agent for use in commercial applications because of its high activity with cephalosporin C and is reasonable resistant to the oxidants O<sub>2 </sub>and H<sub>2</sub>O<sub>2</sub> byproducts of reaction. In this study, response surface methodology (RSM) in shake flask culture was used to enhance the production of DAAO from <i style="mso-bidi-font-style:normal">T. variabilis</i> by optimization of fermentation media composition. The effects of six factors (DL-alanine, glucose, pH, ZnSO<sub>4</sub>, (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> and temperature) were evaluated on DAAO production. Results of Placket-Burman design showed that DL-alanine, pH, glucose and ZnSO<sub>4</sub> were significant factors for DAAO production (P&lt;0.05). The optimum values of media components as predicted by the central composite design were inducer (DL-alanine) concentration 3 g/L, pH 7.7, glucose 17 g/L and ZnSO<sub>4 </sub>34 mg/L. At these optimum values of media composition, maximum production of DAAO was 153 U/g yeast dry weight. Two-fold increase in DAAO production was achieved after optimization of the physical parameters by RSM. </span></span></span>
Description: 272-278
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.49(4) [August 2012]

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