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Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.49 [2012] >
IJBB Vol.49(4) [August 2012] >
| Title: | Optimization of media composition for D-amino acid oxidase production by Trigonopsis variabilis using biostatistical analysis |
| Authors: | Gupta, Neeraj
Gundampati, Ravi Kumar
Debnath, M |
| Keywords: | D-amino acid oxidase
Trigonopsis variabilis
Response surface methodology
Placket-Burman design
Central composite design |
| Issue Date: | Aug-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | D-amino acid
oxidase (DAAO) is biotechnologically relevant enzyme that is used in various
food and pharmaceutical industries. DAAO from the yeast Trigonopsis
variabilis is an important agent for use in commercial applications because of its
high activity with cephalosporin C and is reasonable resistant to the oxidants
O2 and H2O2 byproducts of reaction. In this study, response surface
methodology (RSM) in shake flask culture was used to enhance the production of
DAAO from T. variabilis by
optimization of fermentation media composition. The effects of six factors (DL-alanine, glucose, pH, ZnSO4, (NH4)2SO4
and temperature) were evaluated on DAAO production. Results of Placket-Burman
design showed that DL-alanine, pH, glucose and ZnSO4 were
significant factors for DAAO production (P<0.05). The optimum values of
media components as predicted by the central composite design were inducer
(DL-alanine) concentration 3 g/L, pH 7.7, glucose 17 g/L and ZnSO4 34
mg/L. At these optimum values of media composition, maximum production of DAAO
was 153 U/g yeast dry weight. Two-fold increase in DAAO production was achieved
after optimization of the physical parameters by RSM.
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| Page(s): | 272-278 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.49(4) [August 2012]
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