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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.49 [2012] >
IJBB Vol.49(4) [August 2012] >
| Title: | Potential of antioxidant enzymes in depicting drought tolerance of wheat (Triticum aestivum L.) |
| Authors: | Devi, Rachana
Kaur, Narinder
Gupta, Anil Kumar |
| Keywords: | Antioxidant enzymes
Wheat
Seedling growth
Drought
Triticum aestivum L. |
| Issue Date: | Aug-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In an effort to determine the biochemical
markers for identifying genotypes before sowing for drought tolerance, changes
in activities of antioxidant enzymes were determined in the seedlings of five
drought-tolerant and five
drought-sensitive wheat (Triticum
aestivum L.) genotypes, each with different genetic background growing
under normal and water deficit conditions induced by 6% mannitol. In comparison
with non-stressed seedlings, the catalase (CAT) activity was upregulated by
more than 50% in the roots of water-stressed seedlings in drought-tolerant
genotypes. Water deficit stress also led to the upregulation of ascorbate
peroxidase (APX) in the endosperms and glutathione reductase (GR), CAT and
peroxidase (POD) in the shoots of stressed seedlings in drought-tolerant
genotypes. Superoxide dismutase (SOD) activity was very low in roots and shoots
and showed non-significant increase under water-stress in tolerant genotypes. Out
of five specified enzyme activities (CAT in roots and shoots, APX in
endosperms, GR and POD in shoots), if any three are upregulated in the
specified tissues under water deficit conditions, the genotype is likely to be
drought-tolerant. Wheat seedlings with low GR and APX activities and high POD
activity in shoots with a low ratio of GR activity of shoot to root of
non-stressed seedlings are likely to perform better under rainfed conditions.
The observed data showed that status of antioxidant enzymes could provide a
meaningful tool for depicting drought tolerance of a wheat genotype. |
| Page(s): | 257-265 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.49(4) [August 2012]
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