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Title: A superoxide anion electrochemical sensor based on the direct electrochemistry of superoxide dismutase assembled layer-by-layer at the L-cysteine modified gold electrode
Authors: Yang, Yan
Zhou, Jiawan
Wu, Liang
Zhang, Xiaohua
Chen, Jinhua
Keywords: Superoxide anion;Superoxide dismutase;Electrochemistry;Poly-(diallyldimethylammonium);Sensors;Modified electrodes;Layer-by-layer assembly
Issue Date: Aug-2012
Publisher: NISCAIR-CSIR, India
Abstract: A superoxide anion (O2) biosensor has been developed based on layer-by-layer assembled poly-(diallyldimethylammonium) (PDDA) and superoxide dismutase (SOD) on the L-cysteine (Cys) modified gold (Au) electrode. In 25 mM phosphate buffer solution (pH 7.2), the {SOD/PDDA}n/Cys/Au electrode shows a pair of well-defined and nearly reversible peak at ~ 84 mV vs SCE, confirming the direct electrochemistry of SOD. Based on the bifunctional enzymatic catalytic activities of SOD to the oxidation and reduction of O2, the developed {SOD/PDDA}5/Cys/Au electrode exhibits good analytical characteristics in the detection of O2, such as low detection limit, good stability, reproducibility, selectivity and particularly a wide linear range (0.5 ~ 546 mM).
Page(s): 1057-1063
ISSN: 0975-0975(Online); 0376-4710(Print)
Appears in Collections:IJC-A Vol.51A(08) [August 2012]

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