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Indian Journal of Chemistry -Section A (IJC-A) >
IJC-A Vol.51A [2012] >
IJC-A Vol.51A(08) [August 2012] >
| Title: | A superoxide anion electrochemical sensor based on the direct electrochemistry of superoxide dismutase assembled layer-by-layer at the L-cysteine modified gold electrode |
| Authors: | Yang, Yan
Zhou, Jiawan
Wu, Liang
Zhang, Xiaohua
Chen, Jinhua |
| Keywords: | Superoxide anion
Superoxide dismutase
Electrochemistry
Poly-(diallyldimethylammonium)
Sensors
Modified electrodes
Layer-by-layer assembly |
| Issue Date: | Aug-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A
superoxide anion (O2•–) biosensor has been developed based
on layer-by-layer assembled
poly-(diallyldimethylammonium) (PDDA) and superoxide dismutase (SOD) on the
L-cysteine (Cys) modified gold (Au) electrode. In 25 mM
phosphate buffer solution (pH 7.2),
the {SOD/PDDA}n/Cys/Au electrode shows a pair of well-defined and nearly
reversible peak at ~ 84 mV vs SCE, confirming the direct electrochemistry of
SOD. Based on the bifunctional enzymatic catalytic activities of SOD to the
oxidation and reduction of O2•–, the developed {SOD/PDDA}5/Cys/Au electrode exhibits good analytical characteristics in the
detection of O2•–, such as low detection limit,
good stability, reproducibility, selectivity
and particularly a wide linear range (0.5 ~
546 mM). |
| Page(s): | 1057-1063 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0975(Online); 0376-4710(Print) |
| Source: | IJC-A Vol.51A(08) [August 2012]
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