Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/14531
Title: <span style="font-size:15.0pt;mso-bidi-font-size:11.0pt" lang="EN-US">A superoxide anion electrochemical sensor based on the direct electrochemistry of superoxide dismutase assembled layer-by-layer at the L-cysteine modified gold electrode </span>
Authors: Yang, Yan
Zhou, Jiawan
Wu, Liang
Zhang, Xiaohua
Chen, Jinhua
Keywords: Superoxide anion
Superoxide dismutase
Electrochemistry
Poly-(diallyldimethylammonium)
Sensors
Modified electrodes
Layer-by-layer assembly
Issue Date: Aug-2012
Publisher: NISCAIR-CSIR, India
Abstract: <span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa"="" lang="EN-US">A superoxide anion <span style="mso-bidi-font-weight:bold">(O<sub>2</sub><sup>•<span style="mso-bidi-font-weight:bold">–</span></sup><span style="mso-bidi-font-weight:bold">) biosensor has been developed based on layer-by-layer assembled poly-(diallyldimethylammonium) (PDDA) and superoxide dismutase (SOD) on the L-cysteine (Cys) modified gold (Au) electrode. In 25 m<i style="mso-bidi-font-style:normal">M</i> phosphate buffer solution (<i style="mso-bidi-font-style:normal">p</i>H 7.2), the <span style="mso-bidi-font-weight:bold">{SOD/PDDA}<sub><span style="mso-bidi-font-weight:bold">n</span></sub><span style="mso-bidi-font-weight: bold">/Cys/Au electrode shows a pair of well-defined and nearly reversible peak at ~ 84 mV vs SCE, confirming the direct electrochemistry of SOD. Based on the bifunctional enzymatic catalytic activities of SOD to the oxidation and reduction of <span style="mso-bidi-font-weight:bold">O<sub>2</sub><sup>•<span style="mso-bidi-font-weight:bold">–</span></sup>, the developed <span style="mso-bidi-font-weight:bold">{SOD/PDDA}<sub><span style="mso-bidi-font-weight:bold">5</span></sub><span style="mso-bidi-font-weight: bold">/Cys/Au electrode exhibits good analytical characteristics in the detection of <span style="mso-bidi-font-weight:bold">O<sub>2</sub><sup>•<span style="mso-bidi-font-weight:bold">–</span></sup>, such as low detection limit, good stability, reproducibility, <span style="mso-bidi-font-weight:bold">selectivity and particularly a wide linear range (0.5 ~ 546 <span style="font-size:11.0pt;mso-bidi-font-size:10.0pt; font-family:Symbol;mso-fareast-font-family:" times="" new="" roman";mso-bidi-font-family:="" "times="" roman";mso-ansi-language:en-us;mso-fareast-language:en-us;="" mso-bidi-language:ar-sa"="" lang="EN-US">m<i style="mso-bidi-font-style:normal"><span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-ansi-language:en-us;mso-fareast-language:="" en-us;mso-bidi-language:ar-sa"="" lang="EN-US">M</span></i><span style="font-size: 11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-ansi-language:en-us;mso-fareast-language:en-us;="" mso-bidi-language:ar-sa"="" lang="EN-US">).</span></span></span></span></span></span></span></span></span></span></span></span>
Description: 1057-1063
URI: http://hdl.handle.net/123456789/14531
ISSN: 0975-0975(Online); 0376-4710(Print)
Appears in Collections:IJC-A Vol.51A(08) [August 2012]

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