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|Title:||A new method for determination of ascorbic acid in fruit juices, pharmaceuticals and biological samples|
|Authors:||Janghel, Etesh K|
|Keywords:||Ascorbic acid;Auramine O dyes;Biological Samples;Pharmaceuticals;Spectrophtotometry|
|Abstract:||This study presents a new, selective and accurate indirect spectrophotometric method for determination of L-ascorbic acid in fruit juices, pharmaceuticals and biological samples. Beer’s law is obeyed by ascorbic acid (conc. range, 0.8-6.3 mg) per 25 ml of final solution (0.032-0.252 ppm). The method showed: apparent molar absorptivity, 1.56 × 105 l mol-1cm-1; Sandell’s sensitivity, 0.0024 mg cm-2; limit of detection, 0.0086 mg ml-1; and limit of quantitation, 0.01 mg ml-1. It was satisfactorily applied for determination of ascorbic acid in fruit juices, pharmaceuticals and biological samples. Reliability of method was established by parallel determination against Leuco crystal violet and Rhodamine – B method.|
|ISSN:||0975-1084 (Online); 0022-4456 (Print)|
|Appears in Collections:||JSIR Vol.71(08) [August 2012]|
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