Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/14525
Title: A new method for determination of ascorbic acid in fruit juices, pharmaceuticals and biological samples
Authors: Janghel, Etesh K
Sar, Santosh
Pervez, Y
Keywords: Ascorbic acid
Auramine O dyes
Biological Samples
Pharmaceuticals
Spectrophtotometry
Issue Date: Aug-2012
Publisher: NISCAIR-CSIR, India
Abstract: This study presents a new, selective and accurate indirect spectrophotometric method for determination of L-ascorbic acid in fruit juices, pharmaceuticals and biological samples. Beer’s law is obeyed by ascorbic acid (conc. range, 0.8-6.3 <span style="font-family:Symbol;mso-bidi-font-family:Symbol">mg) per 25 ml of final solution (0.032-0.252 ppm). The method showed: apparent molar absorptivity, 1.56 × 10<sup>5 </sup>l mol<sup>-1</sup>cm<sup>-1</sup>; Sandell’s sensitivity, 0.0024 <span style="font-family:Symbol;mso-bidi-font-family: Symbol">mg cm<sup>-2</sup>; limit of detection, 0.0086 <span style="font-family:Symbol;mso-bidi-font-family:Symbol">mg ml<sup>-1</sup>; and limit of quantitation, 0.01 <span style="font-family:Symbol;mso-bidi-font-family: Symbol">mg ml<sup>-1</sup>. It was satisfactorily applied for determination of ascorbic acid in fruit juices, pharmaceuticals and biological samples. Reliability of method was established by parallel determination against Leuco crystal violet and Rhodamine – B method. </span></span></span></span>
Description: 549-555
URI: http://hdl.handle.net/123456789/14525
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.71(08) [August 2012]

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