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JSIR Vol.71(08) [August 2012] >


Title: A new method for determination of ascorbic acid in fruit juices, pharmaceuticals and biological samples
Authors: Janghel, Etesh K
Sar, Santosh
Pervez, Y
Keywords: Ascorbic acid
Auramine O dyes
Biological Samples
Pharmaceuticals
Spectrophtotometry
Issue Date: Aug-2012
Publisher: NISCAIR-CSIR, India
Abstract: This study presents a new, selective and accurate indirect spectrophotometric method for determination of L-ascorbic acid in fruit juices, pharmaceuticals and biological samples. Beer’s law is obeyed by ascorbic acid (conc. range, 0.8-6.3 mg) per 25 ml of final solution (0.032-0.252 ppm). The method showed: apparent molar absorptivity, 1.56 × 105 l mol-1cm-1; Sandell’s sensitivity, 0.0024 mg cm-2; limit of detection, 0.0086 mg ml-1; and limit of quantitation, 0.01 mg ml-1. It was satisfactorily applied for determination of ascorbic acid in fruit juices, pharmaceuticals and biological samples. Reliability of method was established by parallel determination against Leuco crystal violet and Rhodamine – B method.
Page(s): 549-555
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Source:JSIR Vol.71(08) [August 2012]

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