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JSIR Vol.71 [2012] >
JSIR Vol.71(08) [August 2012] >
| Title: | A new method for determination of ascorbic acid in fruit juices, pharmaceuticals and biological samples |
| Authors: | Janghel, Etesh K
Sar, Santosh
Pervez, Y |
| Keywords: | Ascorbic acid
Auramine O dyes
Biological Samples
Pharmaceuticals
Spectrophtotometry |
| Issue Date: | Aug-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | This
study presents a new, selective and accurate indirect spectrophotometric method
for determination of L-ascorbic acid in fruit juices, pharmaceuticals and
biological samples. Beer’s law is obeyed by ascorbic acid (conc. range, 0.8-6.3
mg) per 25
ml of final solution (0.032-0.252 ppm). The method showed: apparent molar
absorptivity, 1.56 × 105 l mol-1cm-1;
Sandell’s sensitivity, 0.0024 mg cm-2; limit of detection, 0.0086 mg ml-1;
and limit of quantitation, 0.01 mg ml-1. It was satisfactorily applied for
determination of ascorbic acid in fruit juices, pharmaceuticals and biological
samples. Reliability of method was established by parallel determination
against Leuco crystal violet and Rhodamine – B method.
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| Page(s): | 549-555 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Source: | JSIR Vol.71(08) [August 2012]
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