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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(08) [August 2012] >
| Title: | Increase in voltage gated potassium currents of human lymphocytes on culture |
| Authors: | Snekalatha, S
Kanthakumar, Praghalathan |
| Keywords: | Culture
Lymphocyte
Patch clamp
Potassium currents |
| Issue Date: | Aug-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Voltage gated potassium
channels present in T lymphocytes play an important role during lymphocyte
activation. Though an increase in potassium currents has been reported in
activated lymphocytes, changes in potassium currents in culture without
activation by antigen or mitogen has not been reported. The peak potassium
current densities on day 1 and day 5 of culture have been compared in this
study. Peripheral blood mononuclear cells (PBMCs) were separated by density
gradient centrifugation. Lymphocytes were separated from PBMCs by negative
selection using anti-CD14 coated magnetic beads and cultured under appropriate
conditions without antigenic or mitogenic stimulation. Lymphocytes were patched
on day 1 or day 5 of culture. Voltage gated potassium currents were recorded by
whole cell patch clamp technique using a depolarizing protocol. The mean of
peak current densities recorded at +60 mV on day 1 of culture was 228.12± 89.39
pA/pF (n=7) and on day 5 of culture was 468.96 ± 192.07 pA/pF (n=7). The
difference between the current densities on day 1 and day 5 was found to be
significant. Change in electrophysiological characteristics can lead to
functional changes in the lymphocytes and this should be considered
when culturing lymphocytes in vitro for research and clinical use. |
| Page(s): | 587-590 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(08) [August 2012]
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