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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(08) [August 2012] >
| Title: | Alkaline protease production, extraction and characterization from alkaliphilic Bacillus licheniformis KBDL4: A Lonar soda lake isolate |
| Authors: | Pathak, Anupama P
Deshmukh, Kshipra B |
| Keywords: | Alkaline protease
Bacillus licheniformis
Gelatinous coating
Detergent compatibility
Lonar soda lake |
| Issue Date: | Aug-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A bacterium producing an alkaline protease
was isolated from the Lonar soda lake, Buldhana district
(19°58' N; 76°31' E), Maharashtra,
India. The most
appropriate medium for the growth and protease production was composed of (g/L):
casein 10; yeast extract 4; KH2PO4 0.5, K2HPO4
0.5 and CaCl2 0.5. The enzyme showed maximum activity with and
without 5 mM Ca2+ at 70 and 60 oC,
respectively. The enzyme retained 40 and 82% of its initial activity after
heating for 60 min at 60 oC, in absence and presence of 5 mM
CaCl2 respectively. The enzyme remained active and stable at pH 8-12, with an optimum at pH 10. The enzyme showed stability
towards non-ionic and anionic surfactants, and oxidizing agents. It also showed
excellent stability and compatibility with commonly used laundry detergents.
Wash performance analysis revealed that enzyme could effectively remove blood
stains. It also showed decomposition of gelatinous coating on X- ray film. |
| Page(s): | 569-576 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(08) [August 2012]
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