Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/14483
Title: <span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;letter-spacing:-.1pt;mso-ansi-language:EN-GB;mso-fareast-language:EN-US; mso-bidi-language:HI" lang="EN-GB">Alterations in ambient salinity and <i style="mso-bidi-font-style: normal">p</i>H lead to modulation of developmental gene expression in <i style="mso-bidi-font-style:normal">Microhyla ornata</i> (Duméril and Bibron) and <i style="mso-bidi-font-style:normal">Xenopus laevis</i> (Daudin)</span>
Authors: Chougule, Bhagyashri
Asashima, Makoto
Patwardhan, Vidya
Ghaskadbi, Surendra
Keywords: Altered gene expression
Amphibian development
Environmental influence
High salinity
low pH
Issue Date: Aug-2012
Publisher: NISCAIR-CSIR, India
Abstract: <span style="font-size:11.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">Naturally fertilized <i style="mso-bidi-font-style:normal">Microhyla ornata</i> and <i style="mso-bidi-font-style:normal">Xenopus laevis</i> embryos at dorsal lip of blastopore stage were exposed to 0.3, and 0.6% sodium chloride for high salinity treatment and dilute hydrochloric acid and sodium hydroxide for treatment with low and high <i style="mso-bidi-font-style:normal">p</i>H, respectively. <span style="font-size:11.0pt;mso-bidi-font-size:12.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-bidi-font-family:mangal;="" mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-GB">After treatment for different durations, embryos were studied morphologically and using <i style="mso-bidi-font-style:normal">in situ</i> hybridization with selected genes important for normal development and for coping with environmental stress. <span style="font-size:11.0pt; font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" mso-bidi-font-family:mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;="" mso-bidi-language:hi;mso-bidi-font-style:italic"="" lang="EN-GB">Altered s<span style="font-size:11.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-bidi-font-family:mangal;mso-ansi-language:en-gb;="" mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-GB">alinity and <i style="mso-bidi-font-style:normal">p</i>H caused defects in axis formation and neural tube closure, delay in hatching, abnormal swimming of tadpoles and even developmental arrest. This was accompanied by significant decrease in the expression of selected development-regulating genes like <i style="mso-bidi-font-style: normal">goosecoid</i><span style="mso-bidi-font-style:italic"> (required for gastrulation movements), <i style="mso-bidi-font-style:normal">brachyury</i> (mesodermal marker gene), <i style="mso-bidi-font-style:normal">noggin</i> (involved in neural induction), <i style="mso-bidi-font-style:normal">NCAM</i><span style="mso-bidi-font-style:italic"> (required for neural cell adhesion) and <i style="mso-bidi-font-style:normal">MyoD</i><span style="mso-bidi-font-style: italic"> (essential for muscle development), and considerable increase in the transcription of stress response genes <i>hsp30</i> and <i>hsp70</i><span style="mso-bidi-font-style:italic">. Altering the expression of embryonic genes could be one of the mechanisms through which environmental factors influence development of amphibian embryos.</span></span></span></span></span></span></span></span>
Description: 531-541
URI: http://hdl.handle.net/123456789/14483
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.50(08) [August 2012]

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