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Title: Isolation of alkaline lipase from rubber seed — Partial purification, characterization and its potential applications as a detergent additive
Authors: Weerasooriya, M K B
Kumarasinghe, A A N
Keywords: Detergent additive;Lipid stain;Oxidizing agents;Rubber seed lipase;Surfactants;Washing performance
Issue Date: Jul-2012
Publisher: NISCAIR-CSIR, India
Abstract: Lipase of rubber seed has been isolated, characterized and its compatibility with different commercial detergents and surfactants is investigated. The enzyme is found to be compatible with various ionic and non-ionic surfactants, oxidizing agents as well as commercial detergents. Enzyme shows marked activation in the presence of Triton X-100 and Ca 2+, and remarkable resistance towards Tween-80 , Tween-20 and SDS. The best assay conditions observed for this lipase are found to be pH 8.0 and temperature 40ºC. The enzyme is remarkably stable at alkaline pH (8-10) even after 24 h and thermally stable at 30º- 50ºC. Wash performance of commercial detergent for the removal of fatty stains improves on addition of lipase. Enzyme ability to function in alkaline pH (8-10) and at low temperature 30º- 40ºC, resistance towards various surfactants and tolerance to commercial detergents make this lipase a potential additive for detergent formulations.
Page(s): 244-249
ISSN: 0975-0991 (Online); 0971-457X (Print)
Appears in Collections:IJCT Vol.19(4) [July 2012]

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