|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Chemical Technology (IJCT) >
IJCT Vol.19 [2012] >
IJCT Vol.19(4) [July 2012] >
| Title: | Isolation of alkaline lipase from rubber seed — Partial purification, characterization and its potential applications as a detergent additive |
| Authors: | Weerasooriya, M K B
Kumarasinghe, A A N |
| Keywords: | Detergent additive
Lipid stain
Oxidizing agents
Rubber seed lipase
Surfactants
Washing performance |
| Issue Date: | Jul-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Lipase of rubber seed has been isolated, characterized and its
compatibility with different commercial detergents and surfactants is
investigated. The enzyme is found to be compatible with various ionic and
non-ionic surfactants, oxidizing agents as well as commercial detergents.
Enzyme shows marked activation in the presence of Triton X-100 and Ca 2+,
and remarkable resistance towards Tween-80 , Tween-20 and SDS. The best assay
conditions observed for this lipase are found to be pH 8.0 and temperature 40ºC. The enzyme is remarkably stable at
alkaline pH (8-10) even after 24 h
and thermally stable at 30º- 50ºC. Wash performance of commercial detergent for
the removal of fatty stains improves on addition of lipase. Enzyme ability to
function in alkaline pH (8-10) and at
low temperature 30º- 40ºC, resistance
towards various surfactants and tolerance to commercial detergents make this lipase
a potential additive for detergent formulations. |
| Page(s): | 244-249 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0991 (Online); 0971-457X (Print) |
| Source: | IJCT Vol.19(4) [July 2012]
|
|
