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Title: Protection of maize by storage fungi and aflatoxin production using botanicals
Authors: Sharma, Ankita
Sharma, Kanika
Keywords: Aflatoxins
<i>Aspergillus spp.</i>
Medicinal plants
Stored maize
Issue Date: Jun-2012
Publisher: NISCAIR-CSIR, India
Series/Report no.: Int. cl. (2011.01)-A61K 36/00
Abstract: <span style="mso-bidi-font-size:9.0pt;letter-spacing:-.1pt" lang="EN-GB">In the present study the effect of alcoholic, hydroalcoholic and aqueous extracts of <i style="mso-bidi-font-style:normal">Lawsonia inermis </i>Linn. (Henna), <i style="mso-bidi-font-style:normal">Murraya paniculata </i>(Linn.)<i style="mso-bidi-font-style:normal"> </i>Jack<i style="mso-bidi-font-style:normal"> </i>(Orange jessmine)<i style="mso-bidi-font-style:normal"> </i>and <i style="mso-bidi-font-style:normal">Psidium guajava</i> Linn. (Common guava) leaves on <i style="mso-bidi-font-style:normal">Aspergillus flavus</i> and <i style="mso-bidi-font-style:normal">A. parasiticus</i> growth and aflatoxin production and on growth in corn under storage conditions was studied. The extract that most effectively inhibited growth as well as aflatoxin production during <i style="mso-bidi-font-style:normal">in vitro</i> experiments was found to be aqueous leaf extract of <i style="mso-bidi-font-style:normal">L. inermis</i> and <i style="mso-bidi-font-style:normal">M. paniculata.</i> The MIC obtained with <i style="mso-bidi-font-style:normal">L. inermis</i> leaf aqueous extract was 5 mg/mL and 10 mg/mL of culture medium for <i style="mso-bidi-font-style: normal">A. flavus</i> and <i style="mso-bidi-font-style:normal">A. parasiticus,</i> respectively. Whereas it was 8 mg/mL and 9 mg/mL for <i style="mso-bidi-font-style: normal">A. flavus</i> and <i style="mso-bidi-font-style:normal">A. parasiticus,</i> respectively with <i style="mso-bidi-font-style:normal">M. paniculata</i> leaf aqueous extract. A combination of both extracts was also assayed for the MIC, inhibition of aflatoxin production and applied to maize grains for prevention of infection and aflatoxin synthesis in storage conditions. The MIC obtained for combination of both extracts was 4 mg/mL for both test fungi. Aflatoxins were assayed qualitatively and quantitatively by TLC under 365 nm. The MIC of both extracts was found higher (>30 mg/mL) when examined in maize grains. However, concentrations lower than the MIC drastically inhibited the production of aflatoxins in culture medium and also in maize grains. Half of the MIC inhibited 99% of aflatoxins. </span>
Description: 215-221
ISSN: 0976-0512 (Online); 0976-0504 (Print)
Appears in Collections:IJNPR Vol.3(2) [June 2012]

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