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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Natural Products and Resources (IJNPR)
[Formerly Natural Product Radiance (NPR)] >
IJNPR Vol.3 [2012] >
IJNPR Vol.3(2) [June 2012] >
| Title: | Protection of maize by storage fungi and aflatoxin production using botanicals |
| Authors: | Sharma, Ankita
Sharma, Kanika |
| Keywords: | Aflatoxins
Aspergillus spp.
Medicinal plants
Stored maize |
| Issue Date: | Jun-2012 |
| Publisher: | NISCAIR-CSIR, India |
| IPC Code: | Int. cl. (2011.01)-A61K 36/00 |
| Abstract: | In the present study the
effect of alcoholic, hydroalcoholic and aqueous extracts of Lawsonia inermis Linn. (Henna), Murraya paniculata (Linn.) Jack
(Orange jessmine) and Psidium guajava Linn. (Common guava)
leaves on Aspergillus flavus and A. parasiticus growth and aflatoxin
production and on growth in corn under storage conditions was studied. The
extract that most effectively inhibited growth as well as aflatoxin production
during in vitro experiments was found
to be aqueous leaf extract of L. inermis
and M. paniculata. The MIC obtained
with L. inermis leaf aqueous extract
was 5 mg/mL and 10 mg/mL of culture medium for A. flavus and A. parasiticus,
respectively. Whereas it was 8 mg/mL and 9 mg/mL for A. flavus and A. parasiticus,
respectively with M. paniculata leaf
aqueous extract. A combination of both extracts was also assayed for the MIC,
inhibition of aflatoxin production and applied to maize grains for prevention
of infection and aflatoxin synthesis in storage conditions. The MIC obtained
for combination of both extracts was 4 mg/mL for both test fungi. Aflatoxins
were assayed qualitatively and quantitatively by TLC under 365 nm. The MIC of
both extracts was found higher (>30 mg/mL) when examined in maize grains.
However, concentrations lower than the MIC drastically inhibited the production
of aflatoxins in culture medium and also in maize grains. Half of the MIC
inhibited 99% of aflatoxins.
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| Page(s): | 215-221 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0976-0512 (Online); 0976-0504 (Print) |
| Source: | IJNPR Vol.3(2) [June 2012]
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