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Indian Journal of Natural Products and Resources (IJNPR)
[Formerly Natural Product Radiance (NPR)] >
IJNPR Vol.3 [2012] >
IJNPR Vol.3(2) [June 2012] >
| Title: | Screening and characterization of antiviral compounds from Psidium guajava Linn. root bark against white spot syndrome virus |
| Authors: | Velmurugan, S
Babu, M Michael
Punitha, S M J
Viji, V Thanga
Citarasu, T |
| Keywords: | Psidium guajava
Common guava
Antiviral drugs
White Spot Syndrome Virus |
| Issue Date: | Jun-2012 |
| Publisher: | NISCAIR-CSIR, India |
| IPC Code: | Int. cl. (2011.01)-A61K 36/00, A61P 31/12 |
| Abstract: | White Spot Syndrome Virus (WSSV) has been reported to cause severe
mortality and economic loss in shrimp culture industry especially black tiger
shrimp in worldwide. In present study, Psidium
guajava Linn. root bark was serially extracted with hexane, ethyl acetate
and methanol then screened antiviral activity against WSSV by incubating the
extracts with WSSV infected haemolymph of Penaeus
monodon which was propagated earlier. The incubated haemolymph was
intramuscularly injected to the second abdominal segment of Indian white shrimp
Fennerropenaeus indicus and monitored the survival up to 10 days.
Two step PCR detection was performed from the genomic DNA of treated shrimps
using VP 28-WSSV diagnostic PCR primer. The survival and two step PCR
detections revealed that, the ethyl acetate extract effectively suppressed the
WSSV followed by methanol extracts and no antiviral activity was observed in
the hexane extracts. Phytochemical analysis was performed with the active
extracts and further this was purified through silica column chromatography and
Thin Layer Chromatography. The elution was screened again for anti-WSSV
activity and the antiviral active elution of P. guajava were analysed by GC-MS which revealed that the active
elution contains active compounds such as phenol, 2,5-bis(1,1-dimethylethyl),
diethyl phthalate, asarone, phthalic acid, butyldodecyl ester, phytol and
1,2-benzenedicarboxylic acid, mono(2-ethylhexyl) ester. The present study
revealed that there is a possibility for developing new antiviral drugs from P.
guajava against WSSV infection. |
| Page(s): | 208-214 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0976-0512 (Online); 0976-0504 (Print) |
| Source: | IJNPR Vol.3(2) [June 2012]
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