Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/14413
Title: <span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Comparison of cysteine proteases of four laticiferous plants and characterization of <i style="mso-bidi-font-style:normal">Euphorbia nivulia</i> Buch.-Ham. latex glycosylated cysteine peptidase</span>
Authors: Badgujar, Shamkant B
Mahajan, Raghunath T
Keywords: Cysteine protease
<i>Euphorbia nivulia</i>
Latex
Milk clotting
Proteases
Proteolytic activity
Issue Date: Jun-2012
Publisher: NISCAIR-CSIR, India
Series/Report no.: Int. cl. (2011.01)-A61K 36/00
Abstract: <span style="font-size:11.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">Investigations have been carried out on<b style="mso-bidi-font-weight:normal"> </b>proteolytic activities of four laticiferous plants, viz. <i style="mso-bidi-font-style:normal">Calotropis procera </i><span style="mso-bidi-font-style:italic">(Ait.) R. Br. (<i style="mso-bidi-font-style: normal">Ruie</i><span style="mso-bidi-font-style:italic">), <i style="mso-bidi-font-style:normal">Carica papaya</i><span style="mso-bidi-font-style: italic"> Linn. (<i>Papaya</i>), <i style="mso-bidi-font-style:normal">Euphorbia nivulia</i> Buch.-Ham. (<i>Sabar</i>) and <i style="mso-bidi-font-style:normal">Ficus carica</i> Linn. (<i>Anjir</i>) using Hammerstein grade casein and skimmed milk powder. Ratio of milk clotting to proteolytic activity was highest in the latex sample of <i style="mso-bidi-font-style:normal">C. papaya</i> followed by <i style="mso-bidi-font-style:normal">F. carica</i>, <i style="mso-bidi-font-style: normal">E. nivulia</i> and <i style="mso-bidi-font-style:normal">C. procera</i>. The crude enzyme of <i>Sabar</i><i style="mso-bidi-font-style:normal">, <span style="mso-bidi-font-style:italic">Ruie, Papaya </span></i>and <i>Anjir</i> latices exhibit high proteolytic activity with in a <i>p</i>H range from 6.0-7.8, 6.5-8.0, 6.0-7.5 and 6.0-7.0, while maximum milk clotting activity with in a <i>p</i>H range from 6.0-6.5, 6.0-7.0, 6.0-6.5 and 6.5-7.0, respectively. The optimum temperature of proteolytic enzymes of plant latices were recorded over the temperature range 10-80<span style="font-size:11.0pt; font-family:Symbol;mso-ascii-font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-hansi-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi;mso-char-type:symbol;mso-symbol-font-family:symbol"="" lang="EN-GB">°<span style="font-size:11.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-bidi-font-family:mangal;mso-ansi-language:en-gb;="" mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-GB">C at <i>p</i>H 7.0. Whereas, the optimum temperature range of milk clotting protease(s) for milk coagulation <span style="font-size:11.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:batang;mso-bidi-font-family:mangal;mso-ansi-language:="" en-gb;mso-fareast-language:ko;mso-bidi-language:hi;mso-bidi-font-style:italic"="" lang="EN-GB">was<span style="font-size:11.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-bidi-font-family:mangal;mso-ansi-language:en-gb;="" mso-fareast-language:en-us;mso-bidi-language:hi"="" lang="EN-GB"> found in between 45-55<span style="font-size:11.0pt;font-family:Symbol;mso-ascii-font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-hansi-font-family:="" "times="" roman";mso-bidi-font-family:mangal;mso-ansi-language:en-gb;="" mso-fareast-language:en-us;mso-bidi-language:hi;mso-char-type:symbol;="" mso-symbol-font-family:symbol"="" lang="EN-GB">°<span style="font-size:11.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">C. Proteolytic activity of all latices was inhibited by cysteine protease inhibitors. Thus, cysteine protease is a unique property of all plant latices. This article describes comparative accounts of proteolytic activities of four plants and characterization of glycosylated cysteine protease of <i style="mso-bidi-font-style:normal">E. nivulia</i> latex<span style="font-size:11.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" batang;mso-bidi-font-family:mangal;mso-ansi-language:en-gb;mso-fareast-language:="" ko;mso-bidi-language:hi"="" lang="EN-GB">.</span></span></span></span></span></span></span></span></span></span></span>
Description: 152-160
URI: http://hdl.handle.net/123456789/14413
ISSN: 0976-0512 (Online); 0976-0504 (Print)
Appears in Collections:IJNPR Vol.3(2) [June 2012]

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