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Indian Journal of Natural Products and Resources (IJNPR)
[Formerly Natural Product Radiance (NPR)] >
IJNPR Vol.3 [2012] >
IJNPR Vol.3(2) [June 2012] >
| Title: | Comparison of cysteine proteases of four laticiferous plants and characterization of Euphorbia nivulia Buch.-Ham. latex glycosylated cysteine peptidase |
| Authors: | Badgujar, Shamkant B
Mahajan, Raghunath T |
| Keywords: | Cysteine protease
Euphorbia nivulia
Latex
Milk clotting
Proteases
Proteolytic activity |
| Issue Date: | Jun-2012 |
| Publisher: | NISCAIR-CSIR, India |
| IPC Code: | Int. cl. (2011.01)-A61K 36/00 |
| Abstract: | Investigations have been carried out on
proteolytic activities of four laticiferous plants, viz. Calotropis procera (Ait.) R. Br. (Ruie), Carica papaya Linn. (Papaya), Euphorbia
nivulia Buch.-Ham. (Sabar) and Ficus
carica Linn. (Anjir) using Hammerstein grade casein and skimmed milk
powder. Ratio of milk clotting to proteolytic activity was highest in the latex
sample of C. papaya followed by F. carica, E. nivulia and C. procera.
The crude enzyme of Sabar, Ruie, Papaya and Anjir
latices exhibit high proteolytic activity with in a pH range from 6.0-7.8,
6.5-8.0, 6.0-7.5 and 6.0-7.0, while maximum milk clotting activity with in a pH
range from 6.0-6.5, 6.0-7.0, 6.0-6.5 and 6.5-7.0, respectively. The optimum
temperature of proteolytic enzymes of plant latices were recorded over the
temperature range 10-80°C at pH 7.0. Whereas,
the optimum temperature range of milk clotting protease(s) for milk coagulation
was found in between 45-55°C. Proteolytic activity of all latices was inhibited by cysteine protease
inhibitors. Thus, cysteine protease is a unique property of all plant latices.
This article describes comparative accounts of proteolytic activities of four
plants and characterization of glycosylated cysteine protease of E. nivulia latex. |
| Page(s): | 152-160 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0976-0512 (Online); 0976-0504 (Print) |
| Source: | IJNPR Vol.3(2) [June 2012]
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