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Title: Evaluation of in vitro inhibitory effect of selected plants and Shilajit on HIV-Reverse Transcriptase
Authors: Rege, Anuya A
Ambaye, Ramkrishna Y
Deshmukh, Ranjana A
Keywords: Anti-HIV;HIV-reverse transcriptase (RT);Mangroves;Medicinal plants;RT-inhibition;Shilajit
Issue Date: Jun-2012
Publisher: NISCAIR-CSIR, India
IPC Code: Int. cl. (2011.01)-A61K 36/00
Abstract: Three tropical medicinal plants, Ocimum sanctum Linn., Withania somnifera Dunal and Tinospora cordifolia (Willd.) Miers ex Hook. f. & Thoms. and two mangrove medicinal plants, Avicennia officinalis Linn. and Rhizophora mucronata Lam. were taken up in the present study along with Shilajit. Three extracts, petroleum ether, ethanol (successive) and aqueous were prepared from each plant, whereas, only 2 extracts of Shilajit, ethanol (successive) and aqueous were included for the in vitro study. Thus a total of 17 extracts were evaluated for their effect on HIV-reverse transcriptase of 2 clinical isolates, designated as non-antiretroviral therapy (non-ART, from drug naïve patient) and antiretroviral therapy (ART, from drug-treated patient), by reverse transcriptase inhibition assay. The virus stocks were prepared by standard co-cultivation method. Eleven of the 17 extracts showed more than or equal to 50% (≥50%) inhibition of non-ART isolate whereas, only 9 of the 17 extracts showed ≥50% inhibition of ART isolate. In general, Shilajit extracts showed ≥50% inhibition of both the clinical isolates. Furthermore, different combinations of aqueous extracts were assessed for their effect on reverse transcriptase of non-ART isolate. Combination of A. officinalis and Shilajit showed the highest inhibition of viral enzyme. However, the lowest inhibition was noted with combination of W. somnifera and T. cordifolia.
Page(s): 145-151
ISSN: 0976-0512 (Online); 0976-0504 (Print)
Appears in Collections:IJNPR Vol.3(2) [June 2012]

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