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Title: <span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Evaluation of <i style="mso-bidi-font-style:normal">in vitro</i> inhibitory effect of selected plants and <i style="mso-bidi-font-style:normal">Shilajit</i> on HIV-Reverse Transcriptase</span>
Authors: Rege, Anuya A
Ambaye, Ramkrishna Y
Deshmukh, Ranjana A
Keywords: Anti-HIV
HIV-reverse transcriptase (RT)
Medicinal plants
Issue Date: Jun-2012
Publisher: NISCAIR-CSIR, India
Series/Report no.: Int. cl. (2011.01)-A61K 36/00
Abstract: <span style="font-size:11.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">Three tropical medicinal plants, <i style="mso-bidi-font-style:normal">Ocimum sanctum </i><span style="mso-bidi-font-style:italic">Linn., <i style="mso-bidi-font-style:normal">Withania somnifera</i> Dunal and <i style="mso-bidi-font-style:normal">Tinospora cordifolia</i> (Willd.) Miers ex Hook. f. & Thoms. and two mangrove medicinal plants, <i style="mso-bidi-font-style: normal">Avicennia officinalis</i> Linn. and <i style="mso-bidi-font-style:normal">Rhizophora mucronata</i> Lam. were taken up in the present study along with <i style="mso-bidi-font-style:normal">Shilajit</i>. Three extracts, petroleum ether, ethanol (successive) and aqueous were prepared from each plant, whereas, only 2 extracts of <i style="mso-bidi-font-style:normal">Shilajit</i>, ethanol (successive) and aqueous were included for the <i style="mso-bidi-font-style:normal">in vitro</i> study. Thus a total of 17 extracts were evaluated for their effect on HIV-reverse transcriptase of 2 clinical isolates, designated as non-antiretroviral therapy (non-ART, from drug naïve patient) and antiretroviral therapy (ART, from drug-treated patient), by reverse transcriptase inhibition assay. The virus stocks were prepared by standard co-cultivation method. Eleven of the 17 extracts showed more than or equal to 50% (≥50%) inhibition of non-ART isolate whereas, only 9 of the 17 extracts showed ≥50% inhibition of ART isolate. In general, <i style="mso-bidi-font-style: normal">Shilajit</i> extracts showed ≥50% inhibition of both the clinical isolates. Furthermore, different combinations of aqueous extracts were assessed for their effect on reverse transcriptase of non-ART isolate. Combination of <i style="mso-bidi-font-style:normal">A. officinalis</i> and <i style="mso-bidi-font-style: normal">Shilajit</i> showed the highest inhibition of viral enzyme. However, the lowest inhibition was noted with combination of <i style="mso-bidi-font-style: normal">W. somnifera</i> and <i style="mso-bidi-font-style:normal">T. cordifolia</i>.</span></span>
Description: 145-151
ISSN: 0976-0512 (Online); 0976-0504 (Print)
Appears in Collections:IJNPR Vol.3(2) [June 2012]

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