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Indian Journal of Natural Products and Resources (IJNPR)
[Formerly Natural Product Radiance (NPR)] >
IJNPR Vol.3 [2012] >
IJNPR Vol.3(2) [June 2012] >
| Title: | FRAP (Ferric reducing ability of plasma) assay and effect of Diplazium esculentum (Retz) Sw. (a green vegetable of North India) on central nervous system |
| Authors: | Kaushik, Atul
Jijta, Chanderesh
Kaushik, Jeevan J
Zeray, Robel
Ambesajir, Anghesom
Beyene, Lwam |
| Keywords: | Antioxidants
Caffeine
TPTZ
Aqueous extract
Fem
Diplazium esculentum
FRAP |
| Issue Date: | Jun-2012 |
| Publisher: | NISCAIR-CSIR, India |
| IPC Code: | Int. cl. (2011.01)-A61K 36/00, A61P 25/00 |
| Abstract: | The FRAP assay (Ferric
Reducing Ability of Plasma), a simple
test to determine the total antioxidant
power; has been chosen to assess the free radical
scavenging effects of Diplazium
esculentum (Retz) Sw. FRAP assay
depends upon the ferric tripyridyltriazine [Fe (III)-TPTZ]
complex to the ferrous tripyridyltriazine [Fe (II)-TPTZ] by a reductant at low pH. Fe (II)-TPTZ has an intensive
blue color and can be monitored at 593 nm. Antioxidant activity of petroleum ether, chloroform, acetone,
methanol and aqueous extracts was evaluated.
D. esculentum is a well known green leafy
vegetable of North Indian food, therefore, stimulant effect of its aqueous extract on
central nervous system (CNS) was also determined
on mice using the
actophotometer. Results suggested
that water extract (7.6 mM/dry
wt) possesses highest antioxidant activity. CNS stimulant effect of the extract at all doses level
is much higher when compared to the control and standard
caffeine (a known stimulant). Stimulant
effect was significant (P<0.0001 at all doses tested)
and this was also found to
be dose dependent. Thus it can
be concluded that D. esculentum is very rich to scavenge the free radicals and
stimulate the CNS system as compared
to the standard. |
| Page(s): | 228-231 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0976-0512 (Online); 0976-0504 (Print) |
| Source: | IJNPR Vol.3(2) [June 2012]
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