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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(07) [July 2012] >
| Title: | Degradation of bacterial DNA by a natural antimicrobial agent with the help of biomimetic membrane system |
| Authors: | Bhandary, Suman
Chaki, Shaswati
Mukherjee, Sayanti
Das, Sukhen
Mukherjee, Sanjit
Chaudhuri, Keya
Dastidar, Sujata G |
| Keywords: | Agarose gel electrophoresis
Antibacterial activity
Fluorescence anisotropy
Liposomal membrane
van’t Hoff enthalpy change |
| Issue Date: | Jul-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The
antimicrobial efficacy of methylglyoxal (MG) against several gram-negative
bacteria including Escherichia coli has been reported. To determine the
mechanism of action of MG, molecular interactions between lipid and MG within
the liposomal membrane were also investigated. Multilamellar and unilamellar
vesicles were prepared from 1, 2-dipalmitoyl-sn-glycero-3-phosphocholine
(DPPC). The effect of MG on DPPC liposomal membrane was studied by fluorescence
spectroscopy and differential scanning calorimetry. The results indicate that
MG interacts mainly with the DPPC head group that produces a significant
increase in the fluidity of liposomal vesicles, which could be the cause of a
fusion/aggregation effect in microbial cells. The agarose gel electrophoresis
study with the genomic DNA extracted from E.
coli ATCC 25922 revealed that addition of MG could completely degrade this
DNA within 1 h, pointing out to their distinctly high degree of sensitivity
towards MG. Further, the drug was able to cross the cell
membranes, penetrating into the interior of the cell and interacting with DNA
for demonstrating antibacterial activity of MG. |
| Page(s): | 491-496 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(07) [July 2012]
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