NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(07) [July 2012] >


Title: Degradation of bacterial DNA by a natural antimicrobial agent with the help of biomimetic membrane system
Authors: Bhandary, Suman
Chaki, Shaswati
Mukherjee, Sayanti
Das, Sukhen
Mukherjee, Sanjit
Chaudhuri, Keya
Dastidar, Sujata G
Keywords: Agarose gel electrophoresis
Antibacterial activity
Fluorescence anisotropy
Liposomal membrane
van’t Hoff enthalpy change
Issue Date: Jul-2012
Publisher: NISCAIR-CSIR, India
Abstract: The antimicrobial efficacy of methylglyoxal (MG) against several gram-negative bacteria including Escherichia coli has been reported. To determine the mechanism of action of MG, molecular interactions between lipid and MG within the liposomal membrane were also investigated. Multilamellar and unilamellar vesicles were prepared from 1, 2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). The effect of MG on DPPC liposomal membrane was studied by fluorescence spectroscopy and differential scanning calorimetry. The results indicate that MG interacts mainly with the DPPC head group that produces a significant increase in the fluidity of liposomal vesicles, which could be the cause of a fusion/aggregation effect in microbial cells. The agarose gel electrophoresis study with the genomic DNA extracted from E. coli ATCC 25922 revealed that addition of MG could completely degrade this DNA within 1 h, pointing out to their distinctly high degree of sensitivity towards MG. Further, the drug was able to cross the cell membranes, penetrating into the interior of the cell and interacting with DNA for demonstrating antibacterial activity of MG.
Page(s): 491-496
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.50(07) [July 2012]

Files in This Item:

File Description SizeFormat
IJEB 50(7) 491-496.pdf232.05 kBAdobe PDFView/Open
 Current Page Visits: 454 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 510964 since 06-Feb-2009  Last updated on 11-Apr-2014Webmaster: nopr@niscair.res.in