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Title: Assessment of cell damages induced by gamma rays and pulsed electron beam at different dose rates
Authors: Acharya, A
Joseph, P
Bhat, N N
Sanjeev, G
Narayana, Y
Keywords: Pulsed electron beam; -rays
Issue Date: Jul-2012
Publisher: NISCAIR-CSIR, India
Abstract: The analysis of radiation induced micronuclei frequency in a binucleated cell (MN/BNC) is a standard biological end point to determine the cell damage as well as absorbed dose. In the present study, biological end points such as binucleated cells with micronuclei (BNC with MN) and the number of micronuclei per micro-nucleated binucleated cell (MN/(BNC with MN)) have been used to assess the cell damage. Further, the results of BNC with MN and MN/BNC with MN have been compared with those of previously obtained MN/BNC results. Cell damages have been assessed in human blood lymphocytes, exposed to 60Co -rays at a dose rate of 0.033 Gy s-1 and 8 MeV pulsed electron beam at a mean instantaneous dose rate of 2.6  105 Gy s-1. The relative biological effectiveness (RBE) for electrons in comparison with -rays, has been studied. Dosimeters such as fricke and ferrous ammonium benzoic acid and xylenol orange have been used for the dose measurements. The linear-quadratic dose response curves have been observed for both gamma and electrons. However, the relative effectiveness of 8 MeV electrons with that of gamma radiation for MN yield was found to be more than unity for all the biological endpoints studied at a given dose. Present study suggests that the quantification of BNC with MN and MN/(BNC with MN) along with MN/BNC provides additional information, while assessing radiation induced cell damage.
Page(s): 474-477
ISSN: 0975-1041 (Online); 0019-5596 (Print)
Appears in Collections:IJPAP Vol.50(07) [July 2012]

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