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Title: Biosurfactant production by <i>Bacillus subtilis </i>TD4 and <i>Pseudomonas aeruginosa </i>SU7 grown on crude glycerol obtained from biodiesel production plant as sole carbon source
Authors: Saimmai, Atipan
Rukadee, Onkamon
Sobhon, Vorasan
Maneerat, Suppasil
Keywords: Crude glycerol
Microbial oil recovery
Microbial surfactant
Polyaromatic hydrocarbons
Surface tension
Issue Date: Jun-2012
Publisher: NISCAIR-CSIR, India
Abstract: This study presents isolation of biosurfactant (BS)-producing bacteria, <i>Bacillus subtilis </i>TD4 (<i>Bs</i>TD4) and <i>Pseudomonas aeruginosa </i>SU7 (<i>Pa</i>SU7), from palm oil contaminated soil. BS production was done by using a minimal salt medium with used crude glycerol as carbon and commercial monosodium glutamate as nitrogen source. After microbial cultivations at 30°C under optimum conditions, BSs produced by <i>Bs</i>TD4 and <i>Pa</i>SU7 reduced surface tension of culture supernatant to 30 mN/m with BS yield of 2.65 and 2.25 g/l, respectively. BSs could reduce surface tension of pure water to 26.0 and 28.0 mN/m with critical micelle concentration (CMC) of 12 mg/l (<i>Bs</i>TD4) and 20 mg/l (<i>Pa</i>SU7). BS produced by <i>Bs</i>TD4 exhibited higher oil recovery efficiency than that produced by <i>Pa</i>SU7. Both BSs could recover oil more effectively than synthetic surfactants, could enhance solubility of PAHs, and can emulsify various oil and hydrophobic compounds.
Description: 396-406
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.71(06) [June 2012]

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