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JSIR Vol.71 [2012] >
JSIR Vol.71(06) [June 2012] >
| Title: | Biosurfactant production by Bacillus subtilis TD4 and Pseudomonas aeruginosa SU7 grown on crude glycerol obtained from biodiesel production plant as sole carbon source |
| Authors: | Saimmai, Atipan
Rukadee, Onkamon
Sobhon, Vorasan
Maneerat, Suppasil |
| Keywords: | Crude glycerol
Microbial oil recovery
Microbial surfactant
Polyaromatic hydrocarbons
Surface tension |
| Issue Date: | Jun-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | This study presents isolation of biosurfactant
(BS)-producing bacteria, Bacillus subtilis TD4 (BsTD4) and Pseudomonas
aeruginosa SU7 (PaSU7), from palm oil contaminated soil. BS
production was done by using a minimal salt medium with used crude glycerol as
carbon and commercial monosodium glutamate as nitrogen source. After microbial
cultivations at 30°C under optimum conditions, BSs produced by BsTD4 and
PaSU7 reduced surface tension of culture supernatant to 30 mN/m with BS
yield of 2.65 and 2.25 g/l, respectively. BSs could reduce surface tension of
pure water to 26.0 and 28.0 mN/m with critical micelle concentration (CMC) of
12 mg/l (BsTD4) and 20 mg/l (PaSU7). BS produced by BsTD4
exhibited higher oil recovery efficiency than that produced by PaSU7.
Both BSs could recover oil more effectively than synthetic surfactants, could
enhance solubility of PAHs, and can emulsify various oil and hydrophobic
compounds. |
| Page(s): | 396-406 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Source: | JSIR Vol.71(06) [June 2012]
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