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Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.71 [2012] >
JSIR Vol.71(06) [June 2012] >
| Title: | Simultaneous detection of free polyethylene glycol and PEGylated proteins by western blot |
| Authors: | Tiwari, Krishna
Shebannavar, Sunil
Kattavarapu, Krishna
Pokalwar, Santosh
Mishra, Maheshwari
Chauhan, Ugam Kumari S |
| Keywords: | Anti-PEG
Free PEG
GCSF
PEGylation
Western blot |
| Issue Date: | Jun-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | This
study presents detection of free polyethylene glycol (PEG) and conjugated PEG
in western blot assay using anti-PEG rabbit monoclonals specific to methoxy
group of PEG. In a chromogenic western blot assay, detection limit (DL) for
free and PEGylated protein was 2.5 μg and 0.5 μg, respectively. Using
chemiluminescence substrate, DL of PEGylated granulocyte colony stimulating
factor (GCSF) was 100 pg. Assay applied to PEGylation reaction mixture detected
both free and PEGylated protein.
Commercially available
PEGylated products using western blot assay revealed pharmacologically pure
preparations. |
| Page(s): | 392-395 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Source: | JSIR Vol.71(06) [June 2012]
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