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Research Journals >
Indian Journal of Chemical Technology (IJCT) >
IJCT Vol.19 [2012] >
IJCT Vol.19(3) [May 2012] >
| Title: | Spectrophotometric determination of quetiapine fumarate through ion-pair complexation reaction with tropaeolin ooo |
| Authors: | Vinay, K B
Revanasiddappa, H D |
| Keywords: | Quetiapine fumarate
Spectrophotometry
Spiked human urine
Tropaeolin ooo |
| Issue Date: | May-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A sensitive, rapid and reproducible
spectrophotometric method for the determination of quetiapine fumarate (QTF),
an antipsychotic drug, in bulk drug, tablets and human urine has been developed
and validated. The method includes the formation of a 1:2 chloroform
extractable ion-pair complex between the drug and the dye tropaeolin ooo (TP)
in acidic medium (pH 1.83±0.03). The resulting orange-red coloured
ion-pair complex exhibits an absorption maximum at 480 nm. The optimum conditions
for the ion-pair formation have also been established. The method permits the
determination of QTF over a concentration range 2.0 – 20.0 µg mL-1.
The apparent molar absorptivity and Sandell sensitivity values are found to be
2.30 × 104 Lmol-1cm-1 and 0.0264 µg cm-2
respectively. The limits of quantitation and detection values are also
reported. The accuracy and precision results of the method evaluated on
intra-day and inter-day basis are found to be satisfactory. The proposed method
has been successfully applied to the determination of QTF in bulk drug, tablets
and spiked human urine with satisfactory results. The excipients from the
tablets and other basic substances present in urine do not interfere in the
assay. |
| Page(s): | 205-212 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0991 (Online); 0971-457X (Print) |
| Source: | IJCT Vol.19(3) [May 2012]
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