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IJEB Vol.50 [2012] >
IJEB Vol.50(05) [May 2012] >
| Title: | Phenotypic and molecular characterization of indigenous rhizobia nodulating chickpea in India |
| Authors: | Rai, Rhitu
Dash, Prasanta K
Mohapatra, Trilochan
Singh, Aqbal |
| Keywords: | Chickpea
Genetic diversity
Phylogeny
Rhizobia
Symbiotic efficiency |
| Issue Date: | May-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In a combined approach of phenotypic and genotypic characterization, 28
indigenous rhizobial isolates obtained
from different chickpea growing regions in peninsular and northern India were
analyzed for diversity. The field isolates were compared to two reference
strains TAL620 and UPM-Ca142 representing M.
ciceri and M. mediterraneum respectively.
Phenotypic markers such as resistance
to antibiotics, tolerance to salinity, temperature, pH, phosphate solubilization ability, growth rate and also
symbiotic efficiency showed considerable diversity among rhizobial isolates.
Their phenotypic patterns showed adaptations of rhizobial isolates to abiotic
stresses such as heat and salinity. Two salt tolerant strains (1.5% NaCl by T1
and T4) with relatively high symbiotic efficiency and two P-solubilising
strains
(66.7 and 71 mg/ml by T2 and T5) were identified as potential bioinoculants. Molecular profiling by 16S ribosomal DNA
Restriction Fragment Length Polymorphism (RFLP) revealed three clusters at 67%
similarity level. Further, the isolates were differentiated at intraspecific
level by 16S rRNA gene phylogeny. Results assigned all the chickpea rhizobial
field isolates to belong to three different species of Mesorhizobium genus. 46% of the isolates grouped with Mesorhizobium loti and the rest were
identified as M. ciceri and M. mediterraneum, the two species which
have been formerly described as specific chickpea symbionts. This is the first
report on characterization of chickpea nodulating rhizobia covering soils of
both northern and peninsular India.
The collection of isolates, diverse in terms of species and symbiotic
effectiveness holds a vast pool of genetic material which can be effectively used
to yield superior inoculant strains. |
| Page(s): | 340-350 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(05) [May 2012]
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