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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(05) [May 2012] >
| Title: | Effect of supplementation of dermal fibroblasts conditioned medium on expansion of keratinocytes through enhancing attachment |
| Authors: | Chowdhury, S R
Aminuddin, B S
Ruszymah, B H I |
| Keywords: | Dermal fibroblast conditioned medium
Efficiency of cell attachment
Expansion index
Keratinocytes
Tissue engineering |
| Issue Date: | May-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In
the present study in vitro expansion of human keratinocytes by
supplementing dermal fibroblasts conditioned medium (DFCM) has been reported. Effect
of two different DFCM acquired by culturing fibroblasts in
keratinocyte-specific medium (defined keratinocytes serum free medium, DFCM-DKSFM)
and fibroblast-specific serum free medium (F12: DMEM nutrient mix, DFCM-FD)
have been compared. Growth kinetics of keratinocytes in terms of efficiency of
cell attachment, expansion index, apparent specific growth rate and growth
potential at the end of culture was evaluated in culture supplemented with
DFCM-DKSFM and DFCM-FD in comparison with control i.e. DKSFM only. Results
indicated that supplementation of DFCM caused significant increase in
keratinocyte attachment. Efficiency of keratinocyte attachment in culture supplemented
with DFCM-DKSFM was significantly higher compared to those cultured in DFCM-FD and
DKSFM. In addition, the expansion index of keratinocytes in cultures
supplemented with DFCM-DKSFM and DFCM-FD were
3.7 and 2.2 times higher than that of control condition even though the
apparent growth rate and proliferative potential was found significantly lower.
These results suggested that supplementation of DFCM enhanced expansion of
keratinocyte by increasing efficiency of cell attachment, and DFCM-DKSFM provided
suitable condition for in vitro expansion of keratinocytes compared to
DFCM-FD and control condition. |
| Page(s): | 332-339 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(05) [May 2012]
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