NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(05) [May 2012] >

Title: Prokaryotic expression of chicken infectious anemia apoptin protein and characterization of its polyclonal antibodies
Authors: Saxena, Shikha
Kumar, Gandham Ravi
Singh, Prafull
Chaturvedi, Uttara
Saxena, Lovleen
Kumar, Rajiv
Sahoo, Aditya Prasad
Doley, Juwar
Kumar, Amit
Kumar, Sudesh
Tiwari, Ashok K
Keywords: Chicken infectious anemia
Polyclonal sera
Prokaryotic expression
VP3 gene
Issue Date: May-2012
Publisher: NISCAIR-CSIR, India
Abstract: In the present study recombinant VP3 (rVP3) was expressed in E.coli BL21 (DE3) (pLysS) and its polyclonal antibodies were characterized. SDS–PAGE analysis revealed that the expression of recombinant protein was maximum when induced with 1.5 mM IPTG for 6 h at 37ºC. The 6×His-tagged fusion protein was purified on Ni-NTA and confirmed by Western blot using CAV specific antiserum. Rabbits were immunized with purified rVP3 to raise anti-VP3 polyclonal antibodies. Polyclonal serum was tested for specificity and used for confirming expression of VP3 in HeLa cells transfected with pcDNA.cav.vp3 by indirect fluorescent antibody test (IFAT), flow cytometry and Western blot. Available purified rVP3 and polyclonal antibodies against VP3 may be useful to understand its functions which may lead to application of VP3 in cancer therapeutics.
Page(s): 325-331
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.50(05) [May 2012]

Files in This Item:

File Description SizeFormat
IJEB 50(5) 325-331.pdf584.21 kBAdobe PDFView/Open
 Current Page Visits: 131 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 171908 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: