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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(05) [May 2012] >
| Title: | Anti-HCMV and KSHV effect of a trapping ligand antagonist for Herpesvirus-encoded GPCR |
| Authors: | Liu, Hong-Ai
Sun, Han-Xiao
Mo, Xue-Mei
Li, Shi-Yu
Li, Xiu-Ying
Zhang, Guang
Li, Lu |
| Keywords: | Antagonist
Human Cytomegalovirus
Kaposi’s sarcoma-associated herpesvirus ORF74
Peptide H22-LP
Trapping receptor/ligand
US28 |
| Issue Date: | May-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | We have found and synthesized a trapping ligand peptide H22-LP
(the conservative sequence is NAHCALL) from a random phage library according to
the broad-spectrum trapping receptor H22, which derived from the residue 14-35
near the N-terminal region of receptor US28 on HCMV. In this study, we will
evaluate its potential as an efficient antagonist of US28 and the anti-virus
activity, acting as a broad spectrum chemokine receptors antagonist. Stable
expression of US28 and ORF74 in
NIH/3T3 cells were successfully constructed in
vitro. Flow cytomety was used to determine the concentration of Ca2+
induced by H22-LP, and the binding of H22-LP and US28 was confirmed by
enzyme-linked immunosorbent assay (ELISA). Antivirus
activity of H22-LP on HCMV and KSHV was evaluated by anti-virus experiments.
Our data suggest that H22-LP is an effectual antagonist of receptor US28 of
HCMV and ORF74 of KSHV in the transfection assay, and it has potential to
inhibit infection of HCMV and KSHV. These results provide support for the
development of anti-virus strategies based on targeted inhibiting the infection
of herpesvirus. |
| Page(s): | 313-319 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(05) [May 2012]
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