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Title: Genetic analysis of MHC Class II DRB gene in an endangered Jamunapari breed of goats
Authors: Singh, P K
Singh, S V
Singh, M K
Saxena, V K
Singh, A V
Sohal, J S
Keywords: DRB gene
Jamunapari goat
MHC Class II gene
PCR-RFLP
Issue Date: Apr-2012
Publisher: NISCAIR-CSIR, India
Abstract: Present investigation was aimed to analyze the polymorphism of Class II DRB gene region of MHC, using PCR-RFLP with PstI and TaqI restriction enzymes, in endangered Jamunapari goat breed. A total of 203 Jamunapari goats (male-77 & female-126) were included in the study to know the distribution of different genotypes in the population. PstI revealed three genotypes, i.e., PP, Pp and pp, with genotype frequency as 0.054, 0.222 and 0.724, respectively. TaqI also showed TT, Tt and tt genotypes with frequencies of 0.241, 0.527 and 0.232, respectively. The frequencies of P and p alleles were 0.165 and 0.835, while frequencies of T and t alleles were 0.505 and 0.495, respectively. Marked variations were observed in the distribution of PstI and TaqI generated genotypes in the Jamunapari goat population.
Page(s): 220-223
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.11(2) [April 2012]

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