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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(2) [April 2012] >
| Title: | Genetic analysis of MHC Class II DRB gene in an endangered Jamunapari breed of goats |
| Authors: | Singh, P K
Singh, S V
Singh, M K
Saxena, V K
Singh, A V
Sohal, J S |
| Keywords: | DRB gene
Jamunapari goat
MHC Class II gene
PCR-RFLP |
| Issue Date: | Apr-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Present investigation was
aimed to analyze the polymorphism of Class II DRB gene region of MHC, using PCR-RFLP with PstI and TaqI restriction
enzymes, in endangered Jamunapari goat breed. A total of 203 Jamunapari goats
(male-77 & female-126) were included in the study to know the distribution
of different genotypes in the population. PstI
revealed three genotypes, i.e., PP, Pp and pp, with genotype frequency as
0.054, 0.222 and 0.724, respectively. TaqI
also showed TT, Tt and tt genotypes with frequencies of 0.241, 0.527 and 0.232,
respectively. The frequencies of P and p alleles were 0.165 and 0.835, while
frequencies of T and t alleles were 0.505 and 0.495, respectively. Marked
variations were observed in the distribution of PstI and TaqI generated
genotypes in the Jamunapari goat population. |
| Page(s): | 220-223 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(2) [April 2012]
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