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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(2) [April 2012] >
| Title: | Direct organogenesis from leaf explants of Garcinia indica Choisy: An important medicinal plant |
| Authors: | Chauhan, Devendra K
Thakur, A K
Dass, A
Lima, J M
Malik, S K |
| Keywords: | Garcinia indica
Leaf explants
Organogenesis
Regeneration |
| Issue Date: | Apr-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A high frequency plant regeneration protocol
through direct organogenesis has been developed for Garcinia indica Choisy using leaf explants. The leaf explants were
procured from the in vitro developed
shoots raised from the nodal explants comprising of axillary buds. The leaf
explants were cultured on MS medium supplemented with different concentrations
of BAP. The highest number of shoots per explant was obtained on the medium
supplemented with 22.2 μM BAP, while
the lowest number of shoots was recorded on the medium supplemented with 0.45 μM BAP. The highest percentage of rooted
plants was obtained on MS basal medium (MS +0.45 μM BAP) supplemented with 0.81-1.07 μM NAA, while maximum root length was recorded on the medium
supplemented with 0.05 μM NAA. MS
medium supplemented with 0.45 μM BAP
and 0.81 μM NAA was recorded optimal
for rooting of the shoots. The plantlets were hardened and successfully
acclimatized to the open field conditions. |
| Page(s): | 215-219 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(2) [April 2012]
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