Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/14022
Title: High frequency <i>in vitro</i> shoot regeneration of <i>Clitoria ternatea</i> L. affected by different cultural conditions
Authors: Ismail, Najma
Rani, Uzma
Batra, Amla
Keywords: <i>Clitoria ternatea</i>
<i>In vitro</i>
Shoot regeneration
Sucrose
<i>p</i>H
Issue Date: Apr-2012
Publisher: NISCAIR-CSIR, India
Abstract: An efficient <i>in vitro</i> propagation system was established for direct shoot regeneration in <i>Clitoria ternatea</i> L.. The regeneration protocol was standardized by using different explants, viz., nodal, cotyledonary node and shoot tip, on Murashige and Skoog (MS) medium with different concentrations of sucrose (1-6% ), plant growth regulators BA and Kn (1.0-6.0 µ<i>M</i>), and different levels of <i>p</i>H (5.2-6.2). The highest mean number of shoots (18.7±1.0) was obtained from nodal explants on MS medium containing 3% sucrose and 0.8% agar supplemented with BA (5.0 µ<i>M</i>) at <i>p</i>H 5.8. <i>In vitro</i> regenerated and elongated shoots were rooted on ½ MS medium fortified with IBA (2.0 µ<i>M</i>). Complete plantlets were then hardened, acclimatized and transplanted to natural conditions, where they exhibited 80% survivability.
Description: 210-214
URI: http://hdl.handle.net/123456789/14022
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.11(2) [April 2012]

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