|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(2) [April 2012] >
| Title: | High frequency in vitro shoot regeneration of Clitoria ternatea L. affected by different cultural conditions |
| Authors: | Ismail, Najma
Rani, Uzma
Batra, Amla |
| Keywords: | Clitoria ternatea
In vitro
Shoot regeneration
Sucrose
pH |
| Issue Date: | Apr-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | An efficient in vitro propagation
system was established for direct shoot regeneration in Clitoria ternatea L.. The
regeneration protocol was standardized by using different explants, viz., nodal,
cotyledonary node and shoot tip, on Murashige and Skoog (MS) medium with
different concentrations of sucrose (1-6% ), plant growth regulators BA and Kn
(1.0-6.0 µM), and
different levels of pH (5.2-6.2). The highest mean number of shoots (18.7±1.0) was
obtained from nodal explants on MS medium containing 3% sucrose and 0.8% agar
supplemented with BA (5.0 µM) at pH 5.8. In vitro regenerated and elongated shoots were rooted on ½ MS
medium fortified with IBA (2.0 µM). Complete plantlets
were then hardened, acclimatized and transplanted to natural conditions, where
they exhibited 80% survivability. |
| Page(s): | 210-214 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(2) [April 2012]
|
|
