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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(2) [April 2012] >
| Title: | Organogenesis from the cultures of Nothapodytes foetida (Wight) Sleumer raised on TDZ supplemented media |
| Authors: | Tejavathi, D H
Raveesha, H R
Shobha, K |
| Keywords: | Auxin-cytokinin like activity
Callus cultures
Camptothecin
Nothapodytes foetida
Thidiazuron |
| Issue Date: | Apr-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Mature embryos of Nothapodytes foetida (Wight) Sleumer were inoculated on Murashige
and Skoog (MS), modified MS (MMS) and Phillips and Collins (L2) media
supplemented with TDZ either alone or with BAP
and L-glutamine. Both shoot proliferation from the apical region of shoots and
differentiation of shoots from the callus were observed from the cultures
depending on the type of medium and the hormone combination. Proliferation of
shoots from the apical region of shoots and also organogenesis from the callus,
formed at the transition zone of primary root and hypocotyl of the young
seedling, was obtained on L2+TDZ (0.44 µM)+BAP
(2.22 µM)+L-glutamine (0.03 mM). Regeneration from the callus
cultures was reported for the first time. The dual role of TDZ in promoting
proliferation of shoots and shoot differentiation from the callus, along with
beneficial effects of cytokinin and L-glutamine on the morphogenesis, was
discussed. |
| Page(s): | 205-209 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(2) [April 2012]
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