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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(2) [April 2012] >
| Title: | In vitro plant regeneration of wintergreen (Gaultheria fragrantissima Wall.): Assessment of multiple nutrient formulations and cytokinin types |
| Authors: | Ranyaphi, R A
Mao, A A
Borthakur, S K |
| Keywords: | Gaultheria fragrantissima
Medicinal plant
Rapid clonal propagation |
| Issue Date: | Apr-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A
rapid
clonal micropropagation protocol of Gaultheria
fragrantissima Wall. (Ericaceae), an important medicinal plant from
which wintergreen oil is extracted, has
been developed. Mature plants collected
from the field and in vitro raised juvenile seedling were
used as source, and node and apical shoot were used as explants.
Different
basal media
(MS, WPM, RM & MRM) supplemented with cytokinins (BA, Kin and 2iP) at a range of various
concentrations have been
investigated for multiple shoot induction. The 3 cytokinins induce
shoot regeneration from nodal cuttings of in vitro raised
seedlings in all the media. Optimum shoot induction occurred on MRM (modified rhododendron
medium) supplemented with 2iP. After the first 3
cycle of culture with cytokinin, the shoots proliferated on cytokinin free
basal medium. Subculturing the multiple shoots by separating into clumps of 5 shoots on MRM produced an average of 25 new shoots per culture within 8 wk. The in vitro raised microshoots were
successfully rooted on half-strength basal MRM. Of the different substrates
(sand, soil and leaf mold) studied for weaning, sand was the ideal substrate
with 80% survival. Irrigation with half-strength MRM increased the survival
rate and promoted the growth of the plantlets. Over 1000 plantlets were
successfully weaned. |
| Page(s): | 197-204 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(2) [April 2012]
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