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Title: In vitro plant regeneration of wintergreen (Gaultheria fragrantissima Wall.): Assessment of multiple nutrient formulations and cytokinin types
Authors: Ranyaphi, R A
Mao, A A
Borthakur, S K
Keywords: Gaultheria fragrantissima;Medicinal plant;Rapid clonal propagation
Issue Date: Apr-2012
Publisher: NISCAIR-CSIR, India
Abstract: A rapid clonal micropropagation protocol of Gaultheria fragrantissima Wall. (Ericaceae), an important medicinal plant from which wintergreen oil is extracted, has been developed. Mature plants collected from the field and in vitro raised juvenile seedling were used as source, and node and apical shoot were used as explants. Different basal media (MS, WPM, RM & MRM) supplemented with cytokinins (BA, Kin and 2iP) at a range of various concentrations have been investigated for multiple shoot induction. The 3 cytokinins induce shoot regeneration from nodal cuttings of in vitro raised seedlings in all the media. Optimum shoot induction occurred on MRM (modified rhododendron medium) supplemented with 2iP. After the first 3 cycle of culture with cytokinin, the shoots proliferated on cytokinin free basal medium. Subculturing the multiple shoots by separating into clumps of 5 shoots on MRM produced an average of 25 new shoots per culture within 8 wk. The in vitro raised microshoots were successfully rooted on half-strength basal MRM. Of the different substrates (sand, soil and leaf mold) studied for weaning, sand was the ideal substrate with 80% survival. Irrigation with half-strength MRM increased the survival rate and promoted the growth of the plantlets. Over 1000 plantlets were successfully weaned.
Page(s): 197-204
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.11(2) [April 2012]

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