Please use this identifier to cite or link to this item:
Title: <i>In vitro</i> plant regeneration of wintergreen (<i>Gaultheria fragrantissima</i> Wall.): Assessment of multiple nutrient formulations and cytokinin types
Authors: Ranyaphi, R A
Mao, A A
Borthakur, S K
Keywords: <i>Gaultheria fragrantissima</i>
Medicinal plant
Rapid clonal propagation
Issue Date: Apr-2012
Publisher: NISCAIR-CSIR, India
Abstract: A rapid clonal micropropagation protocol of <i>Gaultheria fragrantissima</i> Wall. (Ericaceae), an important medicinal plant from which wintergreen oil is extracted, has been developed. Mature plants collected from the field and <i>in vitro</i> raised juvenile seedling were used as source, and node and apical shoot were used as explants. Different basal media (MS, WPM, RM & MRM) supplemented with cytokinins (BA, Kin and 2iP) at a range of various concentrations have been investigated for multiple shoot induction. The 3 cytokinins induce shoot regeneration from nodal cuttings of <i>in</i> <i>vitro</i> raised seedlings in all the media. Optimum shoot induction occurred on MRM (modified rhododendron medium) supplemented with 2iP. After the first 3 cycle of culture with cytokinin, the shoots proliferated on cytokinin free basal medium. Subculturing the multiple shoots by separating into clumps of 5 shoots on MRM produced an average of 25 new shoots per culture within 8 wk. The <i>in vitro</i> raised microshoots were successfully rooted on half-strength basal MRM. Of the different substrates (sand, soil and leaf mold) studied for weaning, sand was the ideal substrate with 80% survival. Irrigation with half-strength MRM increased the survival rate and promoted the growth of the plantlets. Over 1000 plantlets were successfully weaned.
Description: 197-204
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.11(2) [April 2012]

Files in This Item:
File Description SizeFormat 
IJBT 11(2) 197-204.pdf207.38 kBAdobe PDFView/Open

Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.