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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(2) [April 2012] >
| Title: | Detection of genetically modified cotton seeds using PCR and real-time PCR |
| Authors: | Vidhya, S Shree
Gowda, P H Ramanjini
Yogendra, K N
Ningaraju, T M
Salome, T |
| Keywords: | Detection method
GM cotton seeds
Labeling regulations
PCR
Real-time PCR |
| Issue Date: | Apr-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Detection of genetically modified (GM) crops and products are necessary to
comply with international labeling regulations and to avoid spurious and
unapproved GM planting. DNA based analytical tools involving PCR and real-time
PCR were used to detect GM cotton seeds. Four pairs of primers specific for CaMV 35S, Nos, nptII and cry1Ac genes
were used for the DNA detection of GM crop. The limit of detection in real-time
PCR was found to be 0.1%. Evidently, these two PCR techniques were successful
in detecting transgenes in the DNA of GM cotton seeds. |
| Page(s): | 176-181 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(2) [April 2012]
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