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Title: Detection of genetically modified cotton seeds using PCR and real-time PCR
Authors: Vidhya, S Shree
Gowda, P H Ramanjini
Yogendra, K N
Ningaraju, T M
Salome, T
Keywords: Detection method;GM cotton seeds;Labeling regulations;PCR;Real-time PCR
Issue Date: Apr-2012
Publisher: NISCAIR-CSIR, India
Abstract: Detection of genetically modified (GM) crops and products are necessary to comply with international labeling regulations and to avoid spurious and unapproved GM planting. DNA based analytical tools involving PCR and real-time PCR were used to detect GM cotton seeds. Four pairs of primers specific for CaMV 35S, Nos, nptII and cry1Ac genes were used for the DNA detection of GM crop. The limit of detection in real-time PCR was found to be 0.1%. Evidently, these two PCR techniques were successful in detecting transgenes in the DNA of GM cotton seeds.
Page(s): 176-181
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.11(2) [April 2012]

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