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IJBT Vol.11(2) [April 2012] >


Title: An innovative method of cellulose acetate membrane based isolation of mitochondria and mtDNA extraction from the liver of Duttaphrynus melanostictus (Schneider, 1799)
Authors: Das, Sanjib Kr
Sengupta, Deepanwita
Ghosh, Debopam
Dutta, Debojyoti
Keywords: Cellulose acetate membrane
Duttaphrynus melanostictus
Mitochondria
mtDNA
Issue Date: Apr-2012
Publisher: NISCAIR-CSIR, India
Abstract: Mitochondria isolated by a non-conventional method of membrane filtration were used for mtDNA extraction. This technique allows trapping of mitochondria on cellulose acetate membrane. The procedure does not involve sophisticated instruments and can be performed out of laboratory conditions. The advantages of this procedure are discussed in this paper.
Page(s): 171-175
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.11(2) [April 2012]

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