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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(2) [April 2012] >
| Title: | In silico 3-D structure prediction of H1N1 2009 neuraminidase and comparative analysis of coding statistics of mutated genes |
| Authors: | Sahoo, Bibhuti Bhusan
Sahu, Gopal Krishna |
| Keywords: | H1N1 2009
MODELLER9v7
Neuraminidase
Swiss Model
Verify3D
PROSA |
| Issue Date: | Apr-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The enzyme neuraminidase (NA), coded by H1N1
virus, catalyses the removal of terminal sialic acid from viral and cellular
glycoconjugates. It cleaves the terminal sialic acid on the glycosylated NA
during virus budding to facilitate virus release. The outbreak of Swine flu is
suspected to be caused due to H274Y mutation in the neuraminidase enzyme of
H1N1 2009 strain. The present study involves 3-D structure modeling of mutated
neuraminidase of the strain A/Poland/274/2009 (H1N1) by MODELLER9v7 and
comparative analysis of coding statistics among NA gene of 3 mutated H1N1
strains with GenBank accession numbers GU112751, GU371269 and CY053923. The
analysis of 3-D model revealed that NAs have a common fold characterised by
β-pleated sheet flanked either side by helices. The amino terminal end of
the molecule is occupied by β-α-β motif and carboxy terminal end by β-hairpin
motif. The molecule is characterised by
24 strands and 3 helices. The α1 helix is the longest among the
three helices. The comparative analysis of coding statistics indicates that the
statistical features f1, f5, f6 and f7
have the most discriminating power for the individual recognition of the
mutated neuraminidase genes of H1N1 2009. |
| Page(s): | 148-155 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(2) [April 2012]
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