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IJBT Vol.11(2) [April 2012] >


Title: Analysis of chitinase gene specific transcript accumulation in tea [Camellia sinensis (L.) O. Kuntze] during induced systemic resistance by methyl jasmonate
Authors: Roy, S C
Chakraborty, B N
Keywords: Camellia sinensis
chitinase gene transcript
ISR
RT-PCR
Issue Date: Apr-2012
Publisher: NISCAIR-CSIR, India
Abstract: Chitinase gene (Pr-3) specific transcript accumulation was increased by 5.57% in mature leaf compared to the other in vitro grown tissues in tea (Camellia sinensis) genotype (T383) during induced systemic resistance by methyl jasmonate. Chitinase (Pr-protein) gene specific two primers were used in RT-PCR reaction to measure the differential transcript accumulation in different tissue systems (young leaf, mature leaf, callus and shoot regenerated from somatic embryo). The time-course expression patterns resulted in differential expression of two transcripts (254 & 366 bp) in response to induction. The 366 bp transcript was sequenced bidirectionally and deposited into the GenBank of NCBI (acc.no. EU373553).
Page(s): 142-147
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.11(2) [April 2012]

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