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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(2) [April 2012] >
| Title: | Analysis of chitinase gene specific transcript accumulation in tea [Camellia sinensis (L.) O. Kuntze] during induced systemic resistance by methyl jasmonate |
| Authors: | Roy, S C
Chakraborty, B N |
| Keywords: | Camellia sinensis
chitinase gene transcript
ISR
RT-PCR |
| Issue Date: | Apr-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Chitinase gene
(Pr-3) specific transcript accumulation was increased by 5.57% in mature leaf
compared to the other in vitro grown
tissues in tea (Camellia sinensis)
genotype (T383) during induced systemic resistance by methyl jasmonate.
Chitinase (Pr-protein) gene specific two primers were used in RT-PCR reaction
to measure the differential transcript accumulation in different tissue systems
(young leaf, mature leaf, callus and shoot regenerated from somatic embryo).
The time-course expression patterns resulted in differential expression of two
transcripts (254 & 366 bp) in response to induction. The 366 bp transcript
was sequenced bidirectionally and deposited into the GenBank of NCBI (acc.no.
EU373553). |
| Page(s): | 142-147 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(2) [April 2012]
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