NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(2) [April 2012] >

Title: Isolation of lectin gene and development of resistant Nicotiana tabacum L. against Spodoptera litura
Authors: Singh, Rashmi
Tiwari, Ila Mukul
Jagadeesh, H M
Kansal, Rekha
Gupta, R N
Koundal, K R
Saini, Raman
Keywords: cDNA library
insect bioassay
lectin gene
moth bean
Issue Date: Apr-2012
Publisher: NISCAIR-CSIR, India
Abstract: Attack of insect pests causes a major loss of agricultural produce. Lectin, a plant insecticidal protein, is known to be offering defense against insects either by adversely affecting the survivability and/or by affecting the fecundity of the insects. In the present study, moth bean cDNA library was constructed for isolating a lectin gene. The isolated moth bean lectin (MbL) gene was cloned in a binary vector pBinAR and introduced into tobacco plants by Agrobacterium-mediated transformation method. Molecular analysis confirmed the incorporation of transgene into the transformed tobacco. Insect bioassay of transgenic plants done against Sporodoptera litura under laboratory conditions showed an overall 50% reduction in the larval weight gain.
Page(s): 134-141
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.11(2) [April 2012]

Files in This Item:

File Description SizeFormat
IJBT 11(2) 134-141.pdf417 kBAdobe PDFView/Open
 Current Page Visits: 140 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 165785 since 01-Sep-2015  Last updated on 27-Jun-2016Webmaster: