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Title: Isolation of lectin gene and development of resistant Nicotiana tabacum L. against Spodoptera litura
Authors: Singh, Rashmi
Tiwari, Ila Mukul
Jagadeesh, H M
Kansal, Rekha
Gupta, R N
Koundal, K R
Saini, Raman
Keywords: cDNA library;insect bioassay;lectin gene;moth bean
Issue Date: Apr-2012
Publisher: NISCAIR-CSIR, India
Abstract: Attack of insect pests causes a major loss of agricultural produce. Lectin, a plant insecticidal protein, is known to be offering defense against insects either by adversely affecting the survivability and/or by affecting the fecundity of the insects. In the present study, moth bean cDNA library was constructed for isolating a lectin gene. The isolated moth bean lectin (MbL) gene was cloned in a binary vector pBinAR and introduced into tobacco plants by Agrobacterium-mediated transformation method. Molecular analysis confirmed the incorporation of transgene into the transformed tobacco. Insect bioassay of transgenic plants done against Sporodoptera litura under laboratory conditions showed an overall 50% reduction in the larval weight gain.
Page(s): 134-141
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.11(2) [April 2012]

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