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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.11 [2012] >
IJBT Vol.11(2) [April 2012] >
| Title: | Isolation of lectin gene and development of resistant Nicotiana tabacum L. against Spodoptera litura |
| Authors: | Singh, Rashmi
Tiwari, Ila Mukul
Jagadeesh, H M
Kansal, Rekha
Gupta, R N
Koundal, K R
Saini, Raman |
| Keywords: | cDNA library
insect bioassay
lectin gene
moth bean |
| Issue Date: | Apr-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Attack of insect pests causes a major loss of agricultural produce.
Lectin, a plant insecticidal protein, is known to be
offering defense against insects either by adversely affecting the
survivability and/or by affecting the fecundity of the insects. In
the present study, moth bean cDNA library was constructed for isolating a lectin
gene. The isolated moth bean lectin (MbL)
gene was cloned in a binary vector pBinAR and introduced into tobacco plants by
Agrobacterium-mediated
transformation method. Molecular analysis confirmed the incorporation of
transgene into the transformed tobacco. Insect bioassay of transgenic plants
done against Sporodoptera litura
under laboratory conditions showed an overall 50% reduction in the larval
weight gain. |
| Page(s): | 134-141 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.11(2) [April 2012]
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