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Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.49 [2012] >
IJBB Vol.49(2) [April 2012] >
| Title: | In vitro antioxidant activity of banana (Musa spp. ABB cv. Pisang Awak) |
| Authors: | Darsini, D Teepica Priya
Maheshu, V
Vishnupriya, M
Sasikumar, J M |
| Keywords: | Banana
Pisang Awak
Antioxidant activity
Polyphenols
Flavonoids
Reducing power
DPPH
Hydroxyl Radical
ABTS
high performance thin layer chromatography |
| Issue Date: | Apr-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The methanolic extract of Musa ABB cv Pisang Awak was investigated for the polyphenolic
contents and antioxidant activity. The total phenol and flavonoid contents of
the fruit extract were found to be 120 mg gallic acid equivalents (GAE) and 440
mg quercetin equivalents (QE)/100 g of sample dry weight, respectively. The
antioxidant activity of the Pisang
Awak methanol extract (PAME) (20-500 µg/ml) was determined using
1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing
capacity, 2-2’-azinobis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS) radical
cation decolourization and hydroxyl radical scavenging capacity (OH·). The EC50 values of DPPH, ABTS
and OH· activities of the PAME and butylated hydroxy toluene (BHT) were found
to be 65 and 9 µg/ml, 29 and 6 µg/ml, 36 and 42 µg/ml respectively. The
reducing capacity increased with increasing concentration (31.5-1000 mg/ml) of
the fruit extract and the activity was comparable with the standard BHT. The
high performance thin layer chromatography (HPTLC) analysis of the extract
revealed the presence of polyphenols. The strong and positive correlations were
obtained between total phenol/flavonoid contents (R2 = 0.693-1.0)
and free radical scavenging ability was attributed to the polyphenols as the
major antioxidants. |
| Page(s): | 124-129 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.49(2) [April 2012]
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