Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/13839
Title: Inhibitory effect of antibodies against human chorionic gonadotropin on the growth of colorectal tumour cells
Authors: Khare, Priyanka
Singh, Om
Jain, Swatantra K
Javed, Saleem
Pal, Rahul
Keywords: Colorectal Cancer
Human Chorionic Gondotropin
Immunotherapy of Cancer
Issue Date: Apr-2012
Publisher: NISCAIR-CSIR, India
Abstract: Human chorionic gonadotropin (hCG) was initially believed to be secreted exclusively by the embryo with its primary function being “rescue” of the corpus luteum. However, recently it has been found that the hormone (or its individual subunits) is also secreted by many cancers and that in many cases secretion is associated with poor patient prognosis. In this study, we assessed the presence of hCG in colorectal cancer cells (CCL-253) and evaluated the anti-tumour effects of anti-hCG antibodies <i style="">in vitro</i> and <i style="">in vivo</i>. Anti-hCG antibodies were reactive with CCL-253, as revealed by confocal immunoflourescence microscopy; both cell surface and intracellular expression were observed. Western blot analysis showed that antibodies appeared to interact with several moieties, indicating a level of cross-reactivity. Anti-hCG antiserum specifically reduced the viability of tumor cells and the addition of complement increased <i style="">in vitro</i> anti-tumor effects. In nude mice implanted with CCL-253 cells, administration of anti-hCG antiserum caused a significant reduction in tumor volume; all treated animals survived, while mortality was observed in control animals. Results suggest that anti-hCG antibodies can mediate significant anti-tumor activity both <i style="">in vitro</i> and <i style="">in vivo</i> and lend support to the rationale of anti-hCG immunization in the therapy of gonadotropin- sensitive cancers.
Description: 92-96
URI: http://hdl.handle.net/123456789/13839
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.49(2) [April 2012]

Files in This Item:
File Description SizeFormat 
IJBB 49(2) 92-96.pdf246.14 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.