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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.49 [2012] >
IJBB Vol.49(2) [April 2012] >
| Title: | Cordycepin induces apoptosis in human neuroblastoma SK-N-BE(2)-C and melanoma SK-MEL-2 cells |
| Authors: | Baik, Ji-Sue
Kwon, Haw-Young
Kim, Kyoung-Sook |
| Keywords: | Cordycepin
Apoptosis
SK-N-BE(2)-C Cell
SK-MEL-2 Cell |
| Issue Date: | Apr-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In this study, the effect of cordycepin (3’-deoxyadenosine),
a major component of Cordyceps militaris, an ingredient of traditional Chinese medicine was investigated for the first time on apoptotsis
in human neuroblastoma SK-N-BE(2)-C and
melanoma SK-MEL-2 cells. Cordycepin
significantly inhibited the proliferation of human neuroblastoma SK-N-BE(2)-C and human melanoma
SK-MEL-2 cells with IC50
values of 120 mM and 80 mM, respectively. Cordycepin treatment at
120 mM and 80 mM, respectively, induced apoptosis
in both cells and caused the increase of cell accumulation in a
time-dependent manner at the apoptotic sub-G1 phase, as evidenced
by the flow cytometry (FCM) and annexin
V-fluorescein isothiocyanate (FITC) analyses. Western blot analysis revealed the induction of active caspase-3 and poly(ADP-ribose)polymerase
(PARP) cleavage by cordycepin treatment.
These results suggest that cordycepin is a potential candidate for cancer
therapy of neuroblastoma and melanoma. |
| Page(s): | 86-91 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.49(2) [April 2012]
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