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Title: Rapid screening and cultivation of oleaginous microorganisms
Authors: Gao, Xinlei
Liu, Ye
Chen, Zhongju
Wu, Li
Keywords: Fatty Acid Composition;Mortierella Isabellina CGMCC 3.3410O;Oil Content;Oleaginous Strain;Rapid Determination Method
Issue Date: Apr-2012
Publisher: NISCAIR-CSIR, India
Abstract: Oleaginous microbial strains were cultivated to identify the best oil-producing strain amongst Yarrowia lipolytica (CGMCC 2.1398), Lipomyces starkeyi (CGMCC 2.1608), Rhodosporidium toruloides (CGMCC 2.1389), Mortierella isabellina (CGMCC 3.3410), Cunninghamella blakeleana (CGMCC 3.970), and Mycobacterium QJ311. A method for rapid determination of oil content and fatty acid composition was established to identify the optimum oil-producing strains. This method had a relative standard deviation of 4.09%, an average recovery ratio of 97.09% and a detection limit of 0.1–1.0 g. Mortierella isabellina CGMCC 3.3410 was identified as the best oil-producing strain amongst the six strains tested, with a total biomass of 75 g/10 L and a lipid content of 35%. A rapid screening method of oleaginous microorganisms is discussed for the first time.
Page(s): 282-289
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.50(04) [April 2012]

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