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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(04) [April 2012] >
| Title: | Rapid screening and cultivation of oleaginous microorganisms |
| Authors: | Gao, Xinlei
Liu, Ye
Chen, Zhongju
Wu, Li |
| Keywords: | Fatty Acid Composition
Mortierella Isabellina CGMCC 3.3410O
Oil Content
Oleaginous Strain
Rapid Determination Method |
| Issue Date: | Apr-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Oleaginous microbial strains were cultivated
to identify the best oil-producing strain amongst Yarrowia lipolytica (CGMCC
2.1398), Lipomyces starkeyi (CGMCC 2.1608), Rhodosporidium toruloides
(CGMCC 2.1389), Mortierella isabellina (CGMCC 3.3410), Cunninghamella
blakeleana (CGMCC 3.970), and Mycobacterium QJ311. A method for
rapid determination of oil content and fatty acid composition was established
to identify the optimum oil-producing strains. This method had a relative standard
deviation of 4.09%, an average recovery ratio of 97.09% and a detection limit of 0.1–1.0 g. Mortierella
isabellina CGMCC 3.3410 was identified as the best oil-producing strain amongst the six strains tested, with
a total biomass of 75 g/10 L and a lipid content of 35%. A rapid screening
method of oleaginous microorganisms is discussed for the first time. |
| Page(s): | 282-289 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(04) [April 2012]
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