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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.50 [2012] >
IJEB Vol.50(04) [April 2012] >
| Title: | Mosquito cell line C6/36 shows resistance to Cyt1Aa6 |
| Authors: | Zhang, Lingling
Huang, Enjiong
Tang, Baozhen
Guan, Xiong
Gelbič, Ivan |
| Keywords: | Bacillus Thuringiensis
C6/36 Cells
Indirect Immunofluorescence Assay
Resistance
Receptor Binding |
| Issue Date: | Apr-2012 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | This
study aimed to investigate the resistance mechanism of C6/36 cells to Cyt1Aa6
protein under selection pressure. Receptor binding properties of Cyt1Aa6 toward
sensitive and resistant C6/36 cells were investigated. More sensitive cells
were detected with goat-anti-rabbit-FITC-labeled antibody, and the quantity of in vitro activated Cyt1Aa6 toxin bound
to resistant cells was greatly reduced. Ligand western blot assays showed that
disappearance of the 26 kDa protein and weakness of the positive bands of 68
kDa from resistant cells might lead to the resistance of C6/36 cells to Cyt1Aa6
toxin. The resistance of C6/36 cells
was detected under selection in vitro-activated Cyt1Aa6 toxin. Receptor binding
demonstrated that reduced Cyt1Aa6 bound to resistant cells, which might be
closely related to the disappearance and weakness of some proteins. The results
presented here are the first to demonstrate that Cyt1Aa protein, a uniquely
characteristic toxin, induced resistance at the cellular level. It might be
attributed to the change of receptors. |
| Page(s): | 265-269 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.50(04) [April 2012]
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